TY - JOUR
T1 - 3-MCPD (3-monochloro-1,2-propanediol) inhibit myogenic differentiation in murine skeletal myoblasts
AU - Lee, Hee Seok
AU - Lee, Jaewook
AU - Lee, Seok Hee
AU - Seo, Hyeyeong
AU - Lee, Kwang Geun
AU - Park, Yooheon
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/25
Y1 - 2021/2/25
N2 - 3-Monochloro-1,2-propanediol (3-MCPD) is a chemical compound that is unintentionally produced during food processing such as acid hydrolysis. There has been reports regarding the role of this chemical compound in reproductive toxicity, as well as genotoxicity, neurotoxicity, and kidney toxicity. In this study, the in vitro muscle toxicity of 3-MCPD was assessed using C2C12 myoblast cells. The reduction in muscle regulatory factors (MRFs), which is related to muscle differentiation, was identified as significant with the increase concentration of 3-MCPD. Also, significantly decreased protein expression in mTOR and p70S6 kinase, which are the downstream targets of the pathway associated with muscle synthesis, was also confirmed. Therefore, the inhibitory effect of 3-MCPD on muscle differentiation is considered to be the cause of suppressing mTOR and p70S6 kinase expression. In conclusion, it was confirmed that 3-MCPD inhibits muscle differentiation in C2C12 myoblasts through suppressing the expression of several genetic factors involving muscle differentiation.
AB - 3-Monochloro-1,2-propanediol (3-MCPD) is a chemical compound that is unintentionally produced during food processing such as acid hydrolysis. There has been reports regarding the role of this chemical compound in reproductive toxicity, as well as genotoxicity, neurotoxicity, and kidney toxicity. In this study, the in vitro muscle toxicity of 3-MCPD was assessed using C2C12 myoblast cells. The reduction in muscle regulatory factors (MRFs), which is related to muscle differentiation, was identified as significant with the increase concentration of 3-MCPD. Also, significantly decreased protein expression in mTOR and p70S6 kinase, which are the downstream targets of the pathway associated with muscle synthesis, was also confirmed. Therefore, the inhibitory effect of 3-MCPD on muscle differentiation is considered to be the cause of suppressing mTOR and p70S6 kinase expression. In conclusion, it was confirmed that 3-MCPD inhibits muscle differentiation in C2C12 myoblasts through suppressing the expression of several genetic factors involving muscle differentiation.
KW - 3-Monochloro-1,2-propanediol (3-MCPD)
KW - Differentiated myoblasts
KW - Muscle regulatory factors (MRFs)
UR - http://www.scopus.com/inward/record.url?scp=85100413469&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2020.109311
DO - 10.1016/j.cbi.2020.109311
M3 - Article
C2 - 33171136
AN - SCOPUS:85100413469
SN - 0009-2797
VL - 336
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
M1 - 109311
ER -