TY - JOUR
T1 - 4’-O-β-D-glucosyl-5-O-methylvisamminol attenuates pro-inflammatory responses and protects against oxidative damages
AU - Yoo, Ok Kyung
AU - Keum, Young Sam
N1 - Publisher Copyright:
Copyright © 2019 The Korean Society of Applied Pharmacology.
PY - 2019
Y1 - 2019
N2 - We attempted to examine anti-inflammatory and anti-oxidant effects of 4’-O-β-D-glucosyl-5-O-methylvisamminol (GOMV), the first epigenetic inhibitor of histone phosphorylation at Ser10. While GOMV did not affect the viability of murine macrophage RAW 264.7 cells, it significantly suppressed lipopolysaccharide (LPS)-induced generation of prostaglandin E2 (PGE2) and nitric oxide (NO) through transcriptional inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). GOMV also scavenged free radicals in vitro, increased NF-E2-related factor 2 (NRF2), and activated antioxidant response element (ARE), thereby resulting in the induction of phase II cytoprotective enzymes in human keratinocyte HaCaT cells. Finally, GOMV significantly protected HaCaT cells against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative intracellular damages. Together, our results illustrate that GOMV possesses anti-inflammatory and anti-oxidant activity.
AB - We attempted to examine anti-inflammatory and anti-oxidant effects of 4’-O-β-D-glucosyl-5-O-methylvisamminol (GOMV), the first epigenetic inhibitor of histone phosphorylation at Ser10. While GOMV did not affect the viability of murine macrophage RAW 264.7 cells, it significantly suppressed lipopolysaccharide (LPS)-induced generation of prostaglandin E2 (PGE2) and nitric oxide (NO) through transcriptional inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). GOMV also scavenged free radicals in vitro, increased NF-E2-related factor 2 (NRF2), and activated antioxidant response element (ARE), thereby resulting in the induction of phase II cytoprotective enzymes in human keratinocyte HaCaT cells. Finally, GOMV significantly protected HaCaT cells against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative intracellular damages. Together, our results illustrate that GOMV possesses anti-inflammatory and anti-oxidant activity.
KW - 4’-O-β-D-glucosyl-5-O-methylvisamminol (GOMV)
KW - Antioxidant response element (ARE)
KW - NF-E2-related factor 2 (NRF2)
KW - Reactive oxygen species (ROS)
UR - http://www.scopus.com/inward/record.url?scp=85070896247&partnerID=8YFLogxK
U2 - 10.4062/biomolther.2018.232
DO - 10.4062/biomolther.2018.232
M3 - Article
AN - SCOPUS:85070896247
SN - 1976-9148
VL - 27
SP - 381
EP - 385
JO - Biomolecules and Therapeutics
JF - Biomolecules and Therapeutics
IS - 4
ER -