A cholesterol biosensor based on a bi-enzyme immobilized on conducting poly(thionine) film

Md Mahbubur Rahman, Xiao Bo Li, Jaecheon Kim, Beong Ou Lim, A. J.Saleh Ahammad, Jae Joon Lee

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

A simple and cheap cholesterol biosensor was designed by immobilizing cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) onto a poly(thionine)-modified glassy carbon electrode (GCE/PTH). Being mediated by hydroquinone (HQ), the immobilized HRP exhibited excellent electrocatalytic activity in reducing H2O2, which was produced from cholesterol by the enzymatic reaction of ChOx. The linear detection range for cholesterol was 25-125 μM, with a detection limit (S/N = 3) and a sensitivity of 6.3 μM and 0.18 μA/cm2/μM, respectively, under optimal conditions. The highly reproducible and sensitive GCE/PTH/ChOx/HRP sensor exhibited an interference-free signal for cholesterol detection with excellent recoveries for real sample analysis.

Original languageEnglish
Pages (from-to)536-542
Number of pages7
JournalSensors and Actuators B: Chemical
Volume202
DOIs
StatePublished - 31 Oct 2014

Keywords

  • Cholesterol biosensor
  • Cholesterol oxidase
  • Horseradish peroxidase
  • Hydroquinone
  • Poly(thionine)

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