A combination of CD15/CD10, CD64/CD33, CD16/CD13 or CD11b flow cytometric granulocyte panels is sensitive and specific for diagnosis of myelodysplastic syndrome

Jae Woo Chung, Chan Jeoung Park, Choong Hwan Cha, Young Uk Cho, Seongsoo Jang, Hyun Sook Chi, Eul Ju Seo, Jung Hee Lee, Je Hwan Lee, Kyoo Hyung Lee, Ho Joon Im, Jong Jin Seo

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Flow cytometry (FCM) is a reproducible and objective technique that may be useful in the diagnosisof myelodysplastic syndrome (MDS) by detecting abnormal immunophenotypes specific to MDS. We investigated 5 granulocyte/monocyte panels by FCM to find a sensitive and specific combination of panels in order to discriminate MDS from non-clonal hematologic disorders. Bone marrow aspirates from 35 patients with MDS and 25 patients with non-clonal hematologic disorders were studied. We performed FCM using 5 granulocyte/monocyte panels (CD15/CD10/CD45, CD64/CD33/CD45, CD16/CD13/ CD45, CD16/CD11b/CD45, and CD56/CD19/CD7/CD45) to examine the positive rate in MDS and controls, and to find an optimal combination that maximizes the detection rate of MDS. In MDS, the number of abnormal immunophenotypes per 5 granulocytic and 5 monocytic panels were 2.1±1.2 and 2.2±1.4. The rates were higher than the controls (P< 0.001, respectively). As the number of employed panels increased, the percent values of abnormal immunophenotypes increased (P=0.002). The maximum rate of abnormal immunophenotype was 89.7% in MDS patients, especially 100.0% in normal karyotype, when a combination of three panels, CD15/CD10/CD45, CD64/CD33/CD45, and either CD16/CD13/ CD45 or CD16/CD11b/CD45 was used. This study demonstrates that a combination of CD15/CD10, CD64/CD33, CD16/CD13 or CD11b granulocyte panels in FCM is sensitive and specific for diagnosis of MDS.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalAnnals of Clinical and Laboratory Science
Volume42
Issue number3
StatePublished - 2012

Keywords

  • Flow cytometry
  • Grunulocytic panel
  • Immunophenotypic abnormalities
  • Multiparameter
  • Myelodysplastic syndrome

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