A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Hongje Jang; Young Kwan Kim; Hyun Mi Kwon; Woon Seok Yeo; Dong Eun Kim; Dal Hee Min

doi:10.1002/anie.201001332

A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Hongje Jang, Young Kwan Kim, Hyun Mi Kwon, Woon Seok Yeo, Dong Eun Kim, Dal Hee Min

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

223 Scopus citations

Abstract

(Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

Original language	English
Pages (from-to)	5703-5707
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	33
DOIs	https://doi.org/10.1002/anie.201001332
State	Published - 2 Aug 2010

Keywords

Biosensors
DNA
Fluorescence spectroscopy
Graphene
Viruses

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10.1002/anie.201001332

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abstract = "(Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound fl{\'u}orescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.",

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AU - Kim, Young Kwan

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AU - Yeo, Woon Seok

AU - Kim, Dong Eun

AU - Min, Dal Hee

PY - 2010/8/2

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N2 - (Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

AB - (Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

KW - Biosensors

KW - DNA

KW - Fluorescence spectroscopy

KW - Graphene

KW - Viruses

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A graphene-based platform for the assay of duplex-DNA unwinding by helicase

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Keywords

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