A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Hongje Jang; Young Kwan Kim; Hyun Mi Kwon; Woon Seok Yeo; Dong Eun Kim; Dal Hee Min

doi:10.1002/anie.201001332

A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Hongje Jang
, Young Kwan Kim
, Hyun Mi Kwon
, Woon Seok Yeo
, Dong Eun Kim
, Dal Hee Min

Department of Chemistry

Korea Advanced Institute of Science and Technology
Konkuk University

Research output: Contribution to journal › Article › peer-review

226 Scopus citations

Abstract

(Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

Original language	English
Pages (from-to)	5703-5707
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	33
DOIs	https://doi.org/10.1002/anie.201001332
State	Published - 2 Aug 2010

Keywords

Biosensors
DNA
Fluorescence spectroscopy
Graphene
Viruses

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10.1002/anie.201001332

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title = "A graphene-based platform for the assay of duplex-DNA unwinding by helicase",

abstract = "(Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound fl{\'u}orescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.",

keywords = "Biosensors, DNA, Fluorescence spectroscopy, Graphene, Viruses",

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AU - Jang, Hongje

AU - Kim, Young Kwan

AU - Kwon, Hyun Mi

AU - Yeo, Woon Seok

AU - Kim, Dong Eun

AU - Min, Dal Hee

PY - 2010/8/2

Y1 - 2010/8/2

N2 - (Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

AB - (Figure Presented) Time to unwind: Craphene oxide (CO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. CO selectively binds to unwound flúorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

KW - Biosensors

KW - DNA

KW - Fluorescence spectroscopy

KW - Graphene

KW - Viruses

UR - https://www.scopus.com/pages/publications/77955323564

U2 - 10.1002/anie.201001332

DO - 10.1002/anie.201001332

M3 - Article

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AN - SCOPUS:77955323564

SN - 1433-7851

VL - 49

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 33

ER -

A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Abstract

Keywords

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