A method for selection of restriction enzymes for sdCAPS marker construction

Yesol Jeong; Sunyoung Lee; Inhye Choi; Yongpyo Lim; Yoonkang Hur; Jack E. Staub; Sangmin Chung

doi:10.1111/j.1439-0523.2011.01848.x

A method for selection of restriction enzymes for sdCAPS marker construction

Yesol Jeong, Sunyoung Lee, Inhye Choi, Yongpyo Lim, Yoonkang Hur, Jack E. Staub, Sangmin Chung

Department of Life Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Development of PCR-based markers for single-nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost-effective methodologies for SNP marker development that would enhance the use of SNP-based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer-based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple.

Original language	English
Pages (from-to)	401-403
Number of pages	3
Journal	Plant Breeding
Volume	130
Issue number	3
DOIs	https://doi.org/10.1111/j.1439-0523.2011.01848.x
State	Published - Jun 2011

Keywords

Restriction enzyme selection
SdCAPS
Single-nucleotide polymorphism

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10.1111/j.1439-0523.2011.01848.x

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abstract = "Development of PCR-based markers for single-nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost-effective methodologies for SNP marker development that would enhance the use of SNP-based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer-based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple.",

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AU - Lee, Sunyoung

AU - Choi, Inhye

AU - Lim, Yongpyo

AU - Hur, Yoonkang

AU - Staub, Jack E.

AU - Chung, Sangmin

PY - 2011/6

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AB - Development of PCR-based markers for single-nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost-effective methodologies for SNP marker development that would enhance the use of SNP-based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer-based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple.

KW - Restriction enzyme selection

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A method for selection of restriction enzymes for sdCAPS marker construction

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Keywords

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