A new assay for endonuclease/methyltransferase activities based on graphene oxide

Jieon Lee; Young Kwan Kim; Dal Hee Min

doi:10.1021/ac201298r

A new assay for endonuclease/methyltransferase activities based on graphene oxide

Jieon Lee, Young Kwan Kim, Dal Hee Min

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.

Original language	English
Pages (from-to)	8906-8912
Number of pages	7
Journal	Analytical Chemistry
Volume	83
Issue number	23
DOIs	https://doi.org/10.1021/ac201298r
State	Published - 1 Dec 2011

Access to Document

10.1021/ac201298r

Cite this

@article{b06f1895b57046889a59e6cb96cdebac,

title = "A new assay for endonuclease/methyltransferase activities based on graphene oxide",

abstract = "A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.",

author = "Jieon Lee and Kim, {Young Kwan} and Min, {Dal Hee}",

year = "2011",

month = dec,

day = "1",

doi = "10.1021/ac201298r",

language = "English",

volume = "83",

pages = "8906--8912",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "23",

}

TY - JOUR

T1 - A new assay for endonuclease/methyltransferase activities based on graphene oxide

AU - Lee, Jieon

AU - Kim, Young Kwan

AU - Min, Dal Hee

PY - 2011/12/1

Y1 - 2011/12/1

N2 - A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.

AB - A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.

UR - http://www.scopus.com/inward/record.url?scp=82555170558&partnerID=8YFLogxK

U2 - 10.1021/ac201298r

DO - 10.1021/ac201298r

M3 - Article

C2 - 22066684

AN - SCOPUS:82555170558

SN - 0003-2700

VL - 83

SP - 8906

EP - 8912

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 23

ER -

A new assay for endonuclease/methyltransferase activities based on graphene oxide

Abstract

Access to Document

Other files and links

Fingerprint

Cite this