Abstract
A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
| Original language | English |
|---|---|
| Pages (from-to) | 8906-8912 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 83 |
| Issue number | 23 |
| DOIs | |
| State | Published - 1 Dec 2011 |