A novel chemical compound for inhibition of SARS coronavirus helicase

Jin Moo Lee, Jin Beom Cho, Hee Chul Ahn, Woong Jung, Yong Joo Jeong

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.

Original languageEnglish
Pages (from-to)2070-2073
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number11
DOIs
StatePublished - Nov 2017

Keywords

  • ATP hydrolysis
  • Cytotoxicity
  • DsDNA unwinding
  • Helicase
  • SARS

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