A novel real-time PCR method based on signaling-by-incorporation

Da Rae Kim; Hee Chul Ahn; Won Ja Lee; Dae Ro Ahn

doi:10.1039/c0cc04516k

A novel real-time PCR method based on signaling-by-incorporation

Da Rae Kim
, Hee Chul Ahn
, Won Ja Lee
, Dae Ro Ahn

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

Original language	English
Pages (from-to)	791-793
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	2
DOIs	https://doi.org/10.1039/c0cc04516k
State	Published - 14 Dec 2011

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title = "A novel real-time PCR method based on signaling-by-incorporation",

abstract = "We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.",

author = "Kim, \{Da Rae\} and Ahn, \{Hee Chul\} and Lee, \{Won Ja\} and Ahn, \{Dae Ro\}",

year = "2011",

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AU - Kim, Da Rae

AU - Ahn, Hee Chul

AU - Lee, Won Ja

AU - Ahn, Dae Ro

PY - 2011/12/14

Y1 - 2011/12/14

N2 - We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

AB - We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

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M3 - Article

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AN - SCOPUS:78651390310

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JO - Chemical Communications

JF - Chemical Communications

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A novel real-time PCR method based on signaling-by-incorporation

Abstract

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