A poly(trypan blue)-Modified anodized glassy carbon electrode for the sensitive detection of dopamine in the presence of uric acid and ascorbic acid

Xiao Bo Li, Md Mahbubur Rahman, Chuang Ye Ge, Guang Ri Xu, Jae Joon Lee

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11 Scopus citations

Abstract

A conducting polymeric film of trypan blue (TB) was deposited onto an anodized glassy carbon electrode (AGCE) surface by electropolymerization. The poly(trypan blue)-modified AGCE was used for the determination of dopamine (DA) in the presence of uric acid (UA) and ascorbic acid (AA). The PTB/AGCE exhibits good electrocatalytic behavior for the oxidation of DA in phosphate buffer solution (PBS, pH 7.0). The electrochemical oxidation signals of DA, UA, and AA are well-resolved into three distinct peaks in cyclic voltammograms (CVs) with the anodic peak potential separations (δEpa) of ca. 186, 145, and 331 mV between AA-DA, DA-UA, and AA-UA, respectively. The δ Epa values are large enough to discriminate DA from the interference of AA and UA. A detection limit of ca. 0.36 μM (S/N = 3) was obtained for the sensing of DA with a linear range of 1-40 μM in PBS (pH 7.0). The sensor could successfully determine the concentrations of DA in human urine samples with the recoveries of ca. 97.6-102.6%. This approach provides a simple, easy, sensitive, and selective method for the detection of DA in the presence of AA and UA.

Original languageEnglish
Pages (from-to)B34-B39
JournalJournal of the Electrochemical Society
Volume164
Issue number2
DOIs
StatePublished - 2017

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