A protamine-conjugated gold decorated graphene oxide composite as an electrochemical platform for heparin detection

Arunkumar Rengaraj, Yuvaraj Haldorai, Seoung Kyu Hwang, Eunseon Lee, Mi Wha Oh, Tae Joon Jeon, Young Kyu Han, Yun Suk Huh

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In this study, an effective electrochemical sensor was developed for heparin detection using a protamine-conjugated graphene oxide/gold (GO/Au) composite. Protamine is an antidote that can act as an affinity ligand for heparin. The GO was used as support for signal amplification, and Au nanoparticles (NPs) were employed to immobilize the protamine. This Au NPs also increasing the electron transfer rate and enhancing the signal response during protamine-heparin integration. The proposed affinity sensor had a simple fabrication process, a low detection limit (0.9 nM), a wide linear range (1.9 × 10 −7 M to 1.5 × 10 −9 M), high stability, and high selectivity in the detection of heparin.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalBioelectrochemistry
Volume128
DOIs
StatePublished - Aug 2019

Keywords

  • Electrochemical sensor
  • GO/Au composite
  • Heparin
  • Protamine

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