A role of zinc-finger protein 143 for cancer cell migration and invasion through ZEB1 and E-cadherin in colon cancer cells

A. Rome Paek, Chang Hoon Lee, Hye Jin You

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

To investigate the role of zinc-finger protein 143 in cancer cells, we stably introduced ZNF143 expression knockdown by infecting colon cancer cells with short hairpin (sh) RNA-lentiviral particles against ZNF143 (HCT116 sh-ZNF143). Compared to sh-control cells, HCT116 sh-ZNF143 cells showed faster wound healing, increased migration through Transwell chambers, and increased invasion through Matrigel in Transwell chambers. ZNF143 knockdown increased transcriptional expression of ZEB1. Additionally, ZNF143 regulated E-cadherin transcriptional expression. Small interfering-RNA-mediated silencing of ZEB1 expression affected motility in HCT116 sh-ZNF143 cells. These data suggest that ZNF143 is involved in cellular motility through a ZEB1-E-cadherin-linked pathway in colon cancer cells.

Original languageEnglish
Pages (from-to)E161-E168
JournalMolecular Carcinogenesis
Volume53
Issue numberS1
DOIs
StatePublished - 2014

Keywords

  • E-cadherin
  • Invasion
  • Migration
  • ZEB1
  • ZNF143

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