TY - JOUR
T1 - A shape-code nanoplasmonic biosensor for multiplex detection of Alzheimer's disease biomarkers
AU - Kim, Hanbi
AU - Lee, Jong Uk
AU - Song, Sojin
AU - Kim, Soohyun
AU - Sim, Sang Jun
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/3/15
Y1 - 2018/3/15
N2 - Alzheimer's disease (AD) is a neurodegenerative disease associated with the loss of nerve cells in the brain. The disease is affected by multifactorial pathways and leads to changes in related biomolecular levels as AD progresses. Therefore, AD should be diagnosed with combined detection of several lesions to improve accuracy. Amyloid beta 1–40, 1–42 and τ (tau) protein are milestones in AD pathology and can be used as main screening and diagnostic target markers. Here, we suggest a highly selective biosensor for detection of AD core biomarkers on one platform through distinct localized surface plasmon resonance (LSPR) depending on gold nanoparticles shapes, called a shape-code biosensor. This plasmonic sensor consists of only gold nanoparticles and antibody, but does not need additory methods for precise separation from multifarious samples and identification of markers. Under physiological condition, we determined a detection limit of 34.9 fM for amyloid beta (Aβ) 1–40, 26 fM for Aβ 1–42 and 23.6 fM for τ protein corresponding to the ~ 1, ~ 2.23 and ~ 3.12 nm of Rayleigh scattering peak shift on shape-code plasmon system for each biomarker in mimicked blood. This is the first highly sensitive shape-code biosensor to detect AD biomarkers which can be used to diagnose AD easily in the future.
AB - Alzheimer's disease (AD) is a neurodegenerative disease associated with the loss of nerve cells in the brain. The disease is affected by multifactorial pathways and leads to changes in related biomolecular levels as AD progresses. Therefore, AD should be diagnosed with combined detection of several lesions to improve accuracy. Amyloid beta 1–40, 1–42 and τ (tau) protein are milestones in AD pathology and can be used as main screening and diagnostic target markers. Here, we suggest a highly selective biosensor for detection of AD core biomarkers on one platform through distinct localized surface plasmon resonance (LSPR) depending on gold nanoparticles shapes, called a shape-code biosensor. This plasmonic sensor consists of only gold nanoparticles and antibody, but does not need additory methods for precise separation from multifarious samples and identification of markers. Under physiological condition, we determined a detection limit of 34.9 fM for amyloid beta (Aβ) 1–40, 26 fM for Aβ 1–42 and 23.6 fM for τ protein corresponding to the ~ 1, ~ 2.23 and ~ 3.12 nm of Rayleigh scattering peak shift on shape-code plasmon system for each biomarker in mimicked blood. This is the first highly sensitive shape-code biosensor to detect AD biomarkers which can be used to diagnose AD easily in the future.
KW - Alzheimer's disease (AD)
KW - Gold nanoparticles (AuNPs)
KW - Localized surface plasmon resonance (LSPR)
KW - Multiplex detection
KW - Plasmonic biosensors
KW - Shape-code
UR - http://www.scopus.com/inward/record.url?scp=85031509120&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2017.10.018
DO - 10.1016/j.bios.2017.10.018
M3 - Article
C2 - 29054022
AN - SCOPUS:85031509120
SN - 0956-5663
VL - 101
SP - 96
EP - 102
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -