TY - JOUR
T1 - A spick-and-span approach to the immobilization of horseradish peroxidase on Au nanospheres incorporated with a methionine/graphene biomatrix for the determination of endocrine disruptor bisphenol A
AU - Vilian, A. T.Ezhil
AU - Giribabu, Krishnan
AU - Choe, Sang Rak
AU - Muruganantham, Rethinasabapathy
AU - Lee, Hoomin
AU - Roh, Changhyun
AU - Huh, Yun Suk
AU - Han, Young Kyu
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017
Y1 - 2017
N2 - In the present study, we employ a straightforward, benign strategy to prepare thiol-functionalized reduced graphene oxide (S-RGO) using methionine as the sulphur source and reducing agent. The immobilization of horseradish peroxidase (HRP) over the AuNPs/S-RGO was developed by incorporating AuNPs on the S-RGO surface. The fabricated HRP/AuNPs/S-RGO electrode exhibits a remarkable decrease in the overpotential and a significantly increased oxidation peak current of bisphenol A (BPA) compared with the bare glassy carbon electrode (GCE) and AuNPs/S-RGO electrode. The biosensor shows an excellent amperometric analytical performance with a low detection limit of 2.6 × 10−12 M and a linear range from 2.0 × 10−11 to 1.18 × 10−9 M, with the response time <2 s for BPA. From the results, the apparent Michaelis-Menten constant was calculated as 8.14 nM. The HRP/AuNPs/S-RGO biosensor exhibited faster response, adequate storage stability, inexpensive, simple fabrication with disposability, satisfactory reproducibility and repeatability, and outstanding selectivity. Finally, the constructed biosensor was utilized successfully for detecting BPA in tomato juice and milk samples with acceptable results.
AB - In the present study, we employ a straightforward, benign strategy to prepare thiol-functionalized reduced graphene oxide (S-RGO) using methionine as the sulphur source and reducing agent. The immobilization of horseradish peroxidase (HRP) over the AuNPs/S-RGO was developed by incorporating AuNPs on the S-RGO surface. The fabricated HRP/AuNPs/S-RGO electrode exhibits a remarkable decrease in the overpotential and a significantly increased oxidation peak current of bisphenol A (BPA) compared with the bare glassy carbon electrode (GCE) and AuNPs/S-RGO electrode. The biosensor shows an excellent amperometric analytical performance with a low detection limit of 2.6 × 10−12 M and a linear range from 2.0 × 10−11 to 1.18 × 10−9 M, with the response time <2 s for BPA. From the results, the apparent Michaelis-Menten constant was calculated as 8.14 nM. The HRP/AuNPs/S-RGO biosensor exhibited faster response, adequate storage stability, inexpensive, simple fabrication with disposability, satisfactory reproducibility and repeatability, and outstanding selectivity. Finally, the constructed biosensor was utilized successfully for detecting BPA in tomato juice and milk samples with acceptable results.
KW - Biosensor
KW - Bisphenol A
KW - Gold nanoparticles
KW - Horseradish peroxidase
KW - Reduced graphene oxide
UR - http://www.scopus.com/inward/record.url?scp=85020047203&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.05.122
DO - 10.1016/j.snb.2017.05.122
M3 - Article
AN - SCOPUS:85020047203
SN - 0925-4005
VL - 251
SP - 804
EP - 812
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -