A versatile vector system for multiple gene expression in plants

Sang Min Chung; Ellen L. Frankman; Tzvi Tzfira

doi:10.1016/j.tplants.2005.06.001

A versatile vector system for multiple gene expression in plants

Sang Min Chung
, Ellen L. Frankman
, Tzvi Tzfira

Department of Life Science

Westhampton Beach High School
Stony Brook University
University of Michigan, Ann Arbor

Research output: Contribution to journal › Short survey › peer-review

114 Scopus citations

Abstract

Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.

Original language	English
Pages (from-to)	357-361
Number of pages	5
Journal	Trends in Plant Science
Volume	10
Issue number	8
DOIs	https://doi.org/10.1016/j.tplants.2005.06.001
State	Published - Aug 2005

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10.1016/j.tplants.2005.06.001

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title = "A versatile vector system for multiple gene expression in plants",

abstract = "Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.",

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year = "2005",

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AU - Chung, Sang Min

AU - Frankman, Ellen L.

AU - Tzfira, Tzvi

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N2 - Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.

AB - Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.

UR - https://www.scopus.com/pages/publications/23044439362

U2 - 10.1016/j.tplants.2005.06.001

DO - 10.1016/j.tplants.2005.06.001

M3 - Short survey

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JO - Trends in Plant Science

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A versatile vector system for multiple gene expression in plants

Abstract

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