TY - JOUR
T1 - Adhesion and differentiation of human mesenchymal stem cells on plasma-functionalized graphenes with different feeding gases
AU - Baik, Ku Youn
AU - Choi, Jinsung
AU - Gwon, Hyeseon
AU - Cho, Jaewon
AU - Kim, Yun Ki
AU - Attri, Pankaj
AU - Kim, Un Jeong
AU - Jung, Ranju
PY - 2014/10
Y1 - 2014/10
N2 - Surface functionalisation of graphene layer has been investigated to improve the efficiencies in bio-applications such as stem cell differentiation or protein sensing. Plasma treatment can induce various functionalities on graphene surface simply by changing feeding gas. In this study, the surface chemistry of graphene is modified by plasma treatment using three different feeding gases of H2, N2, and O2. The effects of differential functionalisation are examined by the adsorption of fibronectin and the adhesion, proliferation, and differentiation of human mesenchymal stem cells. Fibronection adsorption is enhanced on the N2 plasma-treated graphene, but it is not clear on the others. hMSCs adhesion is broadened on all of the plasma-treated graphene substrates, especially on the N2 plasma-treated one. H2 plasma treatment enhances cell proliferation and osteogenic differentiation, and N2 and O2 plasma treatments show a tendency of heterogeneous differentiation to osteoblasts and myoblasts. Different responses of stem cell adhesion, proliferation, and differentiation depending on the plasma-treated graphene surface indicate the importance of highly specific surface functionality in stem cell engineering.
AB - Surface functionalisation of graphene layer has been investigated to improve the efficiencies in bio-applications such as stem cell differentiation or protein sensing. Plasma treatment can induce various functionalities on graphene surface simply by changing feeding gas. In this study, the surface chemistry of graphene is modified by plasma treatment using three different feeding gases of H2, N2, and O2. The effects of differential functionalisation are examined by the adsorption of fibronectin and the adhesion, proliferation, and differentiation of human mesenchymal stem cells. Fibronection adsorption is enhanced on the N2 plasma-treated graphene, but it is not clear on the others. hMSCs adhesion is broadened on all of the plasma-treated graphene substrates, especially on the N2 plasma-treated one. H2 plasma treatment enhances cell proliferation and osteogenic differentiation, and N2 and O2 plasma treatments show a tendency of heterogeneous differentiation to osteoblasts and myoblasts. Different responses of stem cell adhesion, proliferation, and differentiation depending on the plasma-treated graphene surface indicate the importance of highly specific surface functionality in stem cell engineering.
UR - http://www.scopus.com/inward/record.url?scp=84905653824&partnerID=8YFLogxK
U2 - 10.1016/j.carbon.2014.05.033
DO - 10.1016/j.carbon.2014.05.033
M3 - Article
AN - SCOPUS:84905653824
SN - 0008-6223
VL - 77
SP - 302
EP - 310
JO - Carbon
JF - Carbon
ER -