Alkaloids and Coumarins with Adiponectin-Secretion-Promoting Activities from the Leaves of Orixa japonica

Piseth Nhoek, Sungjin Ahn, Pisey Pel, Young Mi Kim, Jungmoo Huh, Hyun Woo Kim, Minsoo Noh, Young Won Chin

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Fractionation of a methanol extract of Orixa japonica leaves led to the identification of five new quinoline alkaloids (1, 2, 4, 8, and 9), three new coumarins (15, 17, and 19), and 20 known compounds. The structures were determined by analysis of 1D and 2D NMR spectroscopic data. The absolute configuration of 19 was proposed by electronic circular dichroism calculation. Among the compounds tested in the phenotypic screening to measure adiponectin secretion in human bone marrow mesenchymal stem cells, metabolites 4 and 12 stimulated adiponectin secretions with EC50values of 13.8 and 25.8 μM, respectively. Further PPARγbinding assay and molecular modeling suggested that compounds 4 and 12 are selective PPARγagonists.

Original languageEnglish
Pages (from-to)138-148
Number of pages11
JournalJournal of Natural Products
Volume86
Issue number1
DOIs
StatePublished - 27 Jan 2023

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