TY - JOUR
T1 - Alkaloids and Coumarins with Adiponectin-Secretion-Promoting Activities from the Leaves of Orixa japonica
AU - Nhoek, Piseth
AU - Ahn, Sungjin
AU - Pel, Pisey
AU - Kim, Young Mi
AU - Huh, Jungmoo
AU - Kim, Hyun Woo
AU - Noh, Minsoo
AU - Chin, Young Won
N1 - Publisher Copyright:
© 2023 American Chemical Society. All rights reserved.
PY - 2023/1/27
Y1 - 2023/1/27
N2 - Fractionation of a methanol extract of Orixa japonica leaves led to the identification of five new quinoline alkaloids (1, 2, 4, 8, and 9), three new coumarins (15, 17, and 19), and 20 known compounds. The structures were determined by analysis of 1D and 2D NMR spectroscopic data. The absolute configuration of 19 was proposed by electronic circular dichroism calculation. Among the compounds tested in the phenotypic screening to measure adiponectin secretion in human bone marrow mesenchymal stem cells, metabolites 4 and 12 stimulated adiponectin secretions with EC50values of 13.8 and 25.8 μM, respectively. Further PPARγbinding assay and molecular modeling suggested that compounds 4 and 12 are selective PPARγagonists.
AB - Fractionation of a methanol extract of Orixa japonica leaves led to the identification of five new quinoline alkaloids (1, 2, 4, 8, and 9), three new coumarins (15, 17, and 19), and 20 known compounds. The structures were determined by analysis of 1D and 2D NMR spectroscopic data. The absolute configuration of 19 was proposed by electronic circular dichroism calculation. Among the compounds tested in the phenotypic screening to measure adiponectin secretion in human bone marrow mesenchymal stem cells, metabolites 4 and 12 stimulated adiponectin secretions with EC50values of 13.8 and 25.8 μM, respectively. Further PPARγbinding assay and molecular modeling suggested that compounds 4 and 12 are selective PPARγagonists.
UR - http://www.scopus.com/inward/record.url?scp=85144386066&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.2c00844
DO - 10.1021/acs.jnatprod.2c00844
M3 - Article
C2 - 36529937
AN - SCOPUS:85144386066
SN - 0163-3864
VL - 86
SP - 138
EP - 148
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 1
ER -