Alteration of seed storage protein composition in soybean [ Glycine max (L.) Merrill] mutant lines induced by γ-irradiation mutagenesis

Kyung Jun Lee, Jin Baek Kim, Sang Hoon Kim, Bo Keun Ha, Byung Moo Lee, Si Yong Kang, Dong Sub Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

This study investigated the alteration of seed storage proteins in soybean mutants induced by γ-irradiation. Five soybean cultivars and four landraces were irradiated with 250 Gy of γ rays to induce variability. The seed storage protein profiles of 414 genetic fixed mutants (M 12-M 20) having excellent agricultural traits were analyzed by SDS-PAGE. Among the 414 mutants, 58 were identifed as lacking lipoxygenase, 89 lacking the α′ subunit, 113 lacking the α subunit, and 40 with an altered β subunit. One hundred and forty-nine mutants lacked the A 3 subunit of glycinin. Fifty-four mutants showed higher trypsin inhibitor (TIA) activity, whereas 139 showed lower TIA activity compared to their original cultivars. The selected mutants with low amounts of antinutritional factors such as trypsin inhibitor, lipoxygenase, and α subunit will constitute genetic resources for improving soybean protein quality.

Original languageEnglish
Pages (from-to)12405-12410
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number23
DOIs
StatePublished - 14 Dec 2011

Keywords

  • γ ray
  • mutation
  • SDS-PAGE
  • seed storage protein
  • soybean

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