TY - JOUR
T1 - Alterations in acetylation of histone H4 lysine 8 and trimethylation of lysine 20 associated with lytic gene promoters during kaposi’s sarcoma-associated herpesvirus reactivation
AU - Lim, Sora
AU - Cha, Seho
AU - Jang, Jun Hyeong
AU - Yang, Dahye
AU - Choe, Joonho
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2017 by The Korean Society for Microbiology and Biotechnology.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Kaposi’s sarcoma-associated herpesvirus (KSHV) is associated with formation of Kaposi’s sarcoma, multicentric Castleman’s disease, and primary effusion lymphoma. Replication and transcription activator (RTA) genes are expressed upon reactivation of KSHV, which displays a biphasic life cycle consisting of latent and lytic replication phases. RTA protein expression results in KSHV genome amplification and successive viral lytic gene expression. Transcriptional activity of viral lytic genes is regulated through epigenetic modifications. In Raji cells latently infected with Epstein-Barr virus, various modifications, such as acetylation and methylation, have been identified at specific lysine residues in histone H4 during viral reactivation, supporting the theory that expression of specific lytic genes is controlled by histone modification processes. Data obtained from chromatin immunoprecipitation and quantitative real-time PCR analyses revealed alterations in the H4K8ac and H4K20me3 levels at lytic gene promoters during reactivation. Our results indicate that H4K20me3 is associated with the maintenance of latency, while H4K8ac contributes to KSHV reactivation in infected TREx BCBL-1 RTA cells.
AB - Kaposi’s sarcoma-associated herpesvirus (KSHV) is associated with formation of Kaposi’s sarcoma, multicentric Castleman’s disease, and primary effusion lymphoma. Replication and transcription activator (RTA) genes are expressed upon reactivation of KSHV, which displays a biphasic life cycle consisting of latent and lytic replication phases. RTA protein expression results in KSHV genome amplification and successive viral lytic gene expression. Transcriptional activity of viral lytic genes is regulated through epigenetic modifications. In Raji cells latently infected with Epstein-Barr virus, various modifications, such as acetylation and methylation, have been identified at specific lysine residues in histone H4 during viral reactivation, supporting the theory that expression of specific lytic genes is controlled by histone modification processes. Data obtained from chromatin immunoprecipitation and quantitative real-time PCR analyses revealed alterations in the H4K8ac and H4K20me3 levels at lytic gene promoters during reactivation. Our results indicate that H4K20me3 is associated with the maintenance of latency, while H4K8ac contributes to KSHV reactivation in infected TREx BCBL-1 RTA cells.
KW - Histone H4 lysine 20 trimethylation
KW - Histone H4 lysine 8 acetylation
KW - Histone modification
KW - Kaposi’s sarcoma-associated herpesvirus
KW - Viral reactivation
UR - http://www.scopus.com/inward/record.url?scp=85011022717&partnerID=8YFLogxK
U2 - 10.4014/jmb.1607.07032
DO - 10.4014/jmb.1607.07032
M3 - Article
C2 - 27780949
AN - SCOPUS:85011022717
SN - 1017-7825
VL - 27
SP - 189
EP - 196
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 1
ER -