Abstract
An efficient cell-free translation system was developed by removal of phosphatase localized in the periplasmic space, which hampers the translation reaction by hydrolyzing ATP. S30 extract was prepared from the spheroplast of Escherichia coli, and as much as 40% of ATP-hydrolysis activity of phosphatases could be easily removed by the spheroplast formation. The reaction period of translation using phosphatase-removed S30 extract could be prolonged and protein synthesis was enhanced by more than 30%. Copyright (C) 2000 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 91-96 |
Number of pages | 6 |
Journal | Journal of Microbiological Methods |
Volume | 43 |
Issue number | 2 |
DOIs | |
State | Published - 15 Dec 2000 |
Keywords
- Cell-free translation
- Phosphatase
- Spheroplast