An efficient cell-free protein synthesis system using periplasmic phosphatase-removed S30 extract

Sang Hyeon Kang, Taek Jin Oh, Ryang Guk Kim, Taek Jin Kang, Seung Ha Hwang, Eun Yeol Lee, Cha Yong Choi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

An efficient cell-free translation system was developed by removal of phosphatase localized in the periplasmic space, which hampers the translation reaction by hydrolyzing ATP. S30 extract was prepared from the spheroplast of Escherichia coli, and as much as 40% of ATP-hydrolysis activity of phosphatases could be easily removed by the spheroplast formation. The reaction period of translation using phosphatase-removed S30 extract could be prolonged and protein synthesis was enhanced by more than 30%. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalJournal of Microbiological Methods
Volume43
Issue number2
DOIs
StatePublished - 15 Dec 2000

Keywords

  • Cell-free translation
  • Phosphatase
  • Spheroplast

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