Abstract
Each of the four nucleotides (A, C, G and T) was introduced as the base following the stop codon to investigate the effect of this fourth base on translational termination efficiency during the heterologous expression of human erythropoietin (hEPO) in E. coli. The efficiency of peptide chain termination in E. coli was markedly dependent on the fourth base. The choice of the fourth base was crucial to prevent the expression of undesirable proteins due to the translational infidelity such as frameshifting and stop codon read-through, and translational termination efficiency could be improved with adenosine as the fourth base.
Original language | English |
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Pages (from-to) | 761-764 |
Number of pages | 4 |
Journal | Biotechnology Techniques |
Volume | 13 |
Issue number | 11 |
DOIs | |
State | Published - 1999 |
Keywords
- Erythropoietin
- Escherichia coli
- Termination efficiency
- Translational stop codon