Abstract
Acyltransferase activity of amidase from Bacillus sp. APB-6 was enhanced (24U) by multiple feedings of N-methylacetamide (70mM) into the production medium. Hyperinduced whole resting cells of Bacillus sp. APB-6 corresponding to 4g/L (dry cell weight), when treated with 10mM DTT (dithiothreitol) resulted in 93% molar conversion of acetamide (300mM) to acetohydroxamic acid in presence of hydroxylamine-HCl (800mM) after 30min at 45°C in a 1L reaction mixture. After lyophilization, a 62g powder containing 34% (wtwt-1) acetohydroxamic acid was recovered. This is the first report where DTT has been used to enhance acyltransfer reaction and such high molar conversion (%) of amide to hydroxamates was recorded at 1L scale.
Original language | English |
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Pages (from-to) | 6579-6586 |
Number of pages | 8 |
Journal | Bioresource Technology |
Volume | 102 |
Issue number | 11 |
DOIs | |
State | Published - Jun 2011 |
Keywords
- Acetohydroxamic acid
- Acyltransferase
- Bacillus sp. APB-6
- DTT
- N-methylacetamide