An improved bioprocess for synthesis of acetohydroxamic acid using DTT (dithiothreitol) treated resting cells of Bacillus sp. APB-6

Deepak Pandey, Rajendra Singh, Duni Chand

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Acyltransferase activity of amidase from Bacillus sp. APB-6 was enhanced (24U) by multiple feedings of N-methylacetamide (70mM) into the production medium. Hyperinduced whole resting cells of Bacillus sp. APB-6 corresponding to 4g/L (dry cell weight), when treated with 10mM DTT (dithiothreitol) resulted in 93% molar conversion of acetamide (300mM) to acetohydroxamic acid in presence of hydroxylamine-HCl (800mM) after 30min at 45°C in a 1L reaction mixture. After lyophilization, a 62g powder containing 34% (wtwt-1) acetohydroxamic acid was recovered. This is the first report where DTT has been used to enhance acyltransfer reaction and such high molar conversion (%) of amide to hydroxamates was recorded at 1L scale.

Original languageEnglish
Pages (from-to)6579-6586
Number of pages8
JournalBioresource Technology
Volume102
Issue number11
DOIs
StatePublished - Jun 2011

Keywords

  • Acetohydroxamic acid
  • Acyltransferase
  • Bacillus sp. APB-6
  • DTT
  • N-methylacetamide

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