Analysis of cag-8: A novel poly(Q)-encoding gene in the mouse brain

Jin Woo Ji, Hye Lim Yang, Sun Jung Kim

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5 Scopus citations

Abstract

We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable UTRs are formed by alternative splicing. A strong positive cis-acting element was found in the neuroblastoma × glioma hybrid NG108-15 and human embryonic kidney HEK293 cell lines. The cag-8 protein was localized in the cytosol and in granular bodies within the nucleus. These findings indicate that cag-8 is a novel poly(Q)-encoding gene, the expression of which is confined primarily to the brain.

Original languageEnglish
Pages (from-to)1254-1260
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number4
DOIs
StatePublished - 11 Aug 2006

Keywords

  • 3′-Untranslated region
  • Alternative splicing
  • Mouse brain
  • Poly(Q)
  • Trinucleotide repeat

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