Abstract
We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable UTRs are formed by alternative splicing. A strong positive cis-acting element was found in the neuroblastoma × glioma hybrid NG108-15 and human embryonic kidney HEK293 cell lines. The cag-8 protein was localized in the cytosol and in granular bodies within the nucleus. These findings indicate that cag-8 is a novel poly(Q)-encoding gene, the expression of which is confined primarily to the brain.
Original language | English |
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Pages (from-to) | 1254-1260 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 346 |
Issue number | 4 |
DOIs | |
State | Published - 11 Aug 2006 |
Keywords
- 3′-Untranslated region
- Alternative splicing
- Mouse brain
- Poly(Q)
- Trinucleotide repeat