Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with in-source Fragmentation

Ji Hye Gil; Jongki Hong; Joong Chul Choe; Young Hwan Kim

doi:10.5012/bkcs.2003.24.8.1163

Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with in-source Fragmentation

Ji Hye Gil, Jongki Hong, Joong Chul Choe, Young Hwan Kim

Department of Chemistry

Korea Basic Science Institute

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

Original language	English
Pages (from-to)	1163-1168
Number of pages	6
Journal	Bulletin of the Korean Chemical Society
Volume	24
Issue number	8
DOIs	https://doi.org/10.5012/bkcs.2003.24.8.1163
State	Published - 20 Aug 2003

Keywords

Electrospray ionization (ESI)
Galactolipids
High performance liquid chromatography (HPLC)
In-source fragmentation
Mass spectrometry

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10.5012/bkcs.2003.24.8.1163

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title = "Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with in-source Fragmentation",

abstract = "The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.",

keywords = "Electrospray ionization (ESI), Galactolipids, High performance liquid chromatography (HPLC), In-source fragmentation, Mass spectrometry",

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T1 - Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with in-source Fragmentation

AU - Gil, Ji Hye

AU - Hong, Jongki

AU - Choe, Joong Chul

AU - Kim, Young Hwan

PY - 2003/8/20

Y1 - 2003/8/20

N2 - The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

AB - The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

KW - Electrospray ionization (ESI)

KW - Galactolipids

KW - High performance liquid chromatography (HPLC)

KW - In-source fragmentation

KW - Mass spectrometry

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U2 - 10.5012/bkcs.2003.24.8.1163

DO - 10.5012/bkcs.2003.24.8.1163

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AN - SCOPUS:0344443873

SN - 0253-2964

VL - 24

SP - 1163

EP - 1168

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 8

ER -

Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with in-source Fragmentation

Abstract

Keywords

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