Analysis of sulfonamide and quinolone antibiotic residues in Korean milk using microbial assays and high performance liquid chromatography

Hyun Hee Chung; Jung Bin Lee; Yun Hee Chung; Kwang Geun Lee

doi:10.1016/j.foodchem.2008.07.021

Analysis of sulfonamide and quinolone antibiotic residues in Korean milk using microbial assays and high performance liquid chromatography

Hyun Hee Chung, Jung Bin Lee, Yun Hee Chung, Kwang Geun Lee

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

A new protocol to analyse sulfonamides and quinolones from cows' and goats' milk was developed. Seven antibiotics, sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfamethazine (SMZ), sulfamerazine (SMR), sulfaquinoxaline (SQX), enrofloxacin (ERF), and ciprofloxacin (CIP), were analysed by microbial assays and HPLC. In microbial assays, 21 of 269 samples were screened to have possible antibiotic residues. In HPLC analysis 4 samples were detected to contain SMR (3 samples) and CIP (1 sample). The concentration of SMR in the cows' milk was 12.2 μg kg^-1, which is higher than the LOQ (limit of quantification) and LOD (limit of detection). The levels of SMR and CIP in the other 3 samples were between their LOD and LOQ. Based on this study, we were able to establish an analytical protocol for accurate and robust detection of sulfonamide and quinolone-type antibiotics in milk using two assays.

Original language	English
Pages (from-to)	297-301
Number of pages	5
Journal	Food Chemistry
Volume	113
Issue number	1
DOIs	https://doi.org/10.1016/j.foodchem.2008.07.021
State	Published - 1 Mar 2009

Keywords

Antibiotic residues
High performance liquid chromatography (HPLC)
Microbial assays
Milk
Quinolone
Sulfonamide

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10.1016/j.foodchem.2008.07.021

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title = "Analysis of sulfonamide and quinolone antibiotic residues in Korean milk using microbial assays and high performance liquid chromatography",

abstract = "A new protocol to analyse sulfonamides and quinolones from cows' and goats' milk was developed. Seven antibiotics, sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfamethazine (SMZ), sulfamerazine (SMR), sulfaquinoxaline (SQX), enrofloxacin (ERF), and ciprofloxacin (CIP), were analysed by microbial assays and HPLC. In microbial assays, 21 of 269 samples were screened to have possible antibiotic residues. In HPLC analysis 4 samples were detected to contain SMR (3 samples) and CIP (1 sample). The concentration of SMR in the cows' milk was 12.2 μg kg-1, which is higher than the LOQ (limit of quantification) and LOD (limit of detection). The levels of SMR and CIP in the other 3 samples were between their LOD and LOQ. Based on this study, we were able to establish an analytical protocol for accurate and robust detection of sulfonamide and quinolone-type antibiotics in milk using two assays.",

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author = "Chung, {Hyun Hee} and Lee, {Jung Bin} and Chung, {Yun Hee} and Lee, {Kwang Geun}",

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AU - Lee, Kwang Geun

PY - 2009/3/1

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N2 - A new protocol to analyse sulfonamides and quinolones from cows' and goats' milk was developed. Seven antibiotics, sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfamethazine (SMZ), sulfamerazine (SMR), sulfaquinoxaline (SQX), enrofloxacin (ERF), and ciprofloxacin (CIP), were analysed by microbial assays and HPLC. In microbial assays, 21 of 269 samples were screened to have possible antibiotic residues. In HPLC analysis 4 samples were detected to contain SMR (3 samples) and CIP (1 sample). The concentration of SMR in the cows' milk was 12.2 μg kg-1, which is higher than the LOQ (limit of quantification) and LOD (limit of detection). The levels of SMR and CIP in the other 3 samples were between their LOD and LOQ. Based on this study, we were able to establish an analytical protocol for accurate and robust detection of sulfonamide and quinolone-type antibiotics in milk using two assays.

AB - A new protocol to analyse sulfonamides and quinolones from cows' and goats' milk was developed. Seven antibiotics, sulfamonomethoxine (SMM), sulfadimethoxine (SDM), sulfamethazine (SMZ), sulfamerazine (SMR), sulfaquinoxaline (SQX), enrofloxacin (ERF), and ciprofloxacin (CIP), were analysed by microbial assays and HPLC. In microbial assays, 21 of 269 samples were screened to have possible antibiotic residues. In HPLC analysis 4 samples were detected to contain SMR (3 samples) and CIP (1 sample). The concentration of SMR in the cows' milk was 12.2 μg kg-1, which is higher than the LOQ (limit of quantification) and LOD (limit of detection). The levels of SMR and CIP in the other 3 samples were between their LOD and LOQ. Based on this study, we were able to establish an analytical protocol for accurate and robust detection of sulfonamide and quinolone-type antibiotics in milk using two assays.

KW - Antibiotic residues

KW - High performance liquid chromatography (HPLC)

KW - Microbial assays

KW - Milk

KW - Quinolone

KW - Sulfonamide

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Analysis of sulfonamide and quinolone antibiotic residues in Korean milk using microbial assays and high performance liquid chromatography

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Keywords

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