Abstract
A sensitive and reliable analytical method for exetimibe in human plasma using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and validated for the pharmacokinetic study. Ezetimibe and internal standard, 4-hydroxychalcone, were extracted by liquid-liquid extraction with methyl t-butyl ether. The high performance liquid chromatographic separation was performed on a Phenomenex Luna C 18 column (2.0mm100mm, 3m particles) with a mobile phase of acetonitrile:water=60:40 (v/v). Tandem mass spectrometry was performed in the electrospray ionization (ESI) negative ion mode, using multiple reaction monitoring (MRM) mode for the quantification. The mass transition pairs of m/z 408271 for ezetimibe and m/z 223117 for internal standard were used. The calibration curve was linear in the concentration range of 0.075-20ng/mL for unchanged ezetimibe and 1-200ng/mL for total ezetimibe with the lower limit of quantification of 0.075ng/mL for unchanged ezetimibe and 1ng/mL for total ezetimibe, respectively. The validated method was successfully used to analyze human plasma samples for application in a pharmacokinetic study.
Original language | English |
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Pages (from-to) | 141-152 |
Number of pages | 12 |
Journal | Journal of Liquid Chromatography and Related Technologies |
Volume | 35 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 2012 |
Keywords
- ezetimibe
- HPLC
- human plasma
- LC-MS/MS
- pharmacokinetics
- tandem mass