Analytical LC-MS/MS method for ezetimibe and its application for pharmacokinetic study

Jung Woo Bae, Chang Ik Choi, Sang Hun Park, Choon Gon Jang, Seok Yong Lee

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A sensitive and reliable analytical method for exetimibe in human plasma using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and validated for the pharmacokinetic study. Ezetimibe and internal standard, 4-hydroxychalcone, were extracted by liquid-liquid extraction with methyl t-butyl ether. The high performance liquid chromatographic separation was performed on a Phenomenex Luna C 18 column (2.0mm100mm, 3m particles) with a mobile phase of acetonitrile:water=60:40 (v/v). Tandem mass spectrometry was performed in the electrospray ionization (ESI) negative ion mode, using multiple reaction monitoring (MRM) mode for the quantification. The mass transition pairs of m/z 408271 for ezetimibe and m/z 223117 for internal standard were used. The calibration curve was linear in the concentration range of 0.075-20ng/mL for unchanged ezetimibe and 1-200ng/mL for total ezetimibe with the lower limit of quantification of 0.075ng/mL for unchanged ezetimibe and 1ng/mL for total ezetimibe, respectively. The validated method was successfully used to analyze human plasma samples for application in a pharmacokinetic study.

Original languageEnglish
Pages (from-to)141-152
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume35
Issue number1
DOIs
StatePublished - 1 Jan 2012

Keywords

  • ezetimibe
  • HPLC
  • human plasma
  • LC-MS/MS
  • pharmacokinetics
  • tandem mass

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