Anti-biofilm activity of N-Mannich bases of berberine linking piperazine against Listeria monocytogenes

Joo Won Kim, Bhupendra Mistry, Han Seung Shin, Seok Seong Kang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In the food processing environment, Listeria monocytogenes has the ability to persist on the surfaces, resulting in serious safety concerns. This study aimed to determine the anti-biofilm activity of N-Mannich bases of berberine linking piperazine (MBP) against L. monocytogenes. Among the MBPs used in this study, MBP5 and MBP6 (200 μg/mL) showed a significant decrease in the L. monocytogenes biofilm on the surface of polystyrene at low (4 °C) and high (37 °C) temperatures (approximately ≥ 50% reduction). Both MBPs also reduced the L. monocytogenes biofilm on the surface of stainless steel at low and high temperatures (approximately ≥ 1 log CFU/cm2 reduction). According to fluorescence and scanning electron microscopic analyses, both MBPs prevented the clustered and aggregated forms of L. monocytogenes, which are typical biofilm characteristics; thus, suggesting that both MBPs effectively reduced the L. monocytogenes biofilm. In addition, XTT reduction and ATP production clearly showed that the viability of L. monocytogenes was obviously decreased in the presence of MBP5 and MBP6. Collectively, these results suggest that MBP5 and MBP6 might be used as promising anti-biofilm agents to prevent the L. monocytogenes biofilm.

Original languageEnglish
Article number107668
JournalFood Control
Volume121
DOIs
StatePublished - Mar 2021

Keywords

  • Bacterial growth
  • Biofilm
  • Listeria monocytogenes
  • N-Mannich bases of berberine linking piperazine

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