TY - JOUR
T1 - Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes
AU - Min, Kim
AU - Jee, Seung Cheol
AU - Sung, Jung Suk
AU - Kadam, Avinash A.
N1 - Publisher Copyright:
© 2018
PY - 2018/10/15
Y1 - 2018/10/15
N2 - Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3O4) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3O4-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3O4-HNTs-CS (termed as Fe3O4-HNTs-CS-Lac). Then free laccase and Fe3O4-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe3O4-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe3O4-HNTs-CS. Therefore, investigated Fe3O4-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe3O4-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.
AB - Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3O4) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3O4-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3O4-HNTs-CS (termed as Fe3O4-HNTs-CS-Lac). Then free laccase and Fe3O4-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe3O4-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe3O4-HNTs-CS. Therefore, investigated Fe3O4-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe3O4-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.
KW - Anti-proliferative laccase
KW - Halloysite nanotubes
KW - Super-magnetic drug carrier
KW - Trametes versicolor laccase
UR - http://www.scopus.com/inward/record.url?scp=85048767981&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2018.06.074
DO - 10.1016/j.ijbiomac.2018.06.074
M3 - Article
C2 - 29913193
AN - SCOPUS:85048767981
SN - 0141-8130
VL - 118
SP - 228
EP - 237
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -