Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

Kim Min; Seung Cheol Jee; Jung Suk Sung; Avinash A. Kadam

doi:10.1016/j.ijbiomac.2018.06.074

Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

Kim Min
, Seung Cheol Jee
, Jung Suk Sung
, Avinash A. Kadam

Department of Life Science

Dongguk University

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe₃O₄) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe₃O₄-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe₃O₄-HNTs-CS (termed as Fe₃O₄-HNTs-CS-Lac). Then free laccase and Fe₃O₄-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe₃O₄-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe₃O₄-HNTs-CS. Therefore, investigated Fe₃O₄-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe₃O₄-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.

Original language	English
Pages (from-to)	228-237
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	118
DOIs	https://doi.org/10.1016/j.ijbiomac.2018.06.074
State	Published - 15 Oct 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anti-proliferative laccase
Halloysite nanotubes
Super-magnetic drug carrier
Trametes versicolor laccase

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10.1016/j.ijbiomac.2018.06.074

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title = "Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes",

abstract = "Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3O4) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3O4-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3O4-HNTs-CS (termed as Fe3O4-HNTs-CS-Lac). Then free laccase and Fe3O4-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe3O4-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe3O4-HNTs-CS. Therefore, investigated Fe3O4-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe3O4-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.",

keywords = "Anti-proliferative laccase, Halloysite nanotubes, Super-magnetic drug carrier, Trametes versicolor laccase",

author = "Kim Min and Jee, \{Seung Cheol\} and Sung, \{Jung Suk\} and Kadam, \{Avinash A.\}",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

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day = "15",

doi = "10.1016/j.ijbiomac.2018.06.074",

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T1 - Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

AU - Min, Kim

AU - Jee, Seung Cheol

AU - Sung, Jung Suk

AU - Kadam, Avinash A.

PY - 2018/10/15

Y1 - 2018/10/15

N2 - Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3O4) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3O4-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3O4-HNTs-CS (termed as Fe3O4-HNTs-CS-Lac). Then free laccase and Fe3O4-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe3O4-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe3O4-HNTs-CS. Therefore, investigated Fe3O4-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe3O4-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.

AB - Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3O4) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3O4-HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3O4-HNTs-CS (termed as Fe3O4-HNTs-CS-Lac). Then free laccase and Fe3O4-HNTs-CS-Lac were evaluated for anti-proliferative properties against cancer cell lines of liver (HepG2), lung (H460), cervix (Hela) and stomach (AGS). Laccase and Fe3O4-HNTs-CS-Lac gave significant cytotoxicity against all studied cancer cell lines. Moreover, the apoptosis analysis and FE-SEM morphology observations of cells support the anti-proliferative potential of laccase immobilized on Fe3O4-HNTs-CS. Therefore, investigated Fe3O4-HNTs-CS-Lac is natural and super-magnetic nano-biocatalyst, having the significant anti-proliferative potential and furthermore, Fe3O4-HNTs-CS can be used as efficient and specific delivery system for other anti-cancer enzymes.

KW - Anti-proliferative laccase

KW - Halloysite nanotubes

KW - Super-magnetic drug carrier

KW - Trametes versicolor laccase

UR - https://www.scopus.com/pages/publications/85048767981

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SN - 0141-8130

VL - 118

SP - 228

EP - 237

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Anti-proliferative applications of laccase immobilized on super-magnetic chitosan-functionalized halloysite nanotubes

Abstract

UN SDGs

Keywords

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