TY - JOUR
T1 - Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India
AU - Kumar, Sanjeet
AU - Mahanti, Padma
AU - Singh, Nihar Ranjan
AU - Rath, Sakti Kant
AU - Jena, Padan Kumar
AU - Patra, Jayanta Kumar
N1 - Publisher Copyright:
© 2017, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was compared with antibiotics Penicillin and Kanamycin. It was observed that DP1 showed significant inhibitory activity against the tested bacteria. The characterization of DP1 through NMR analysis and presence of proton in carbon position at C-3, C-19, C-18, C-21 and C-27 was same as the known compound “Diosgenin”. Therefore, isolated compound was confirmed to be Diosgenin. The study for the first time showed that, diosgenin present in D. pentaphylla tuber was responsible for antibacterial and antioxidant potential. Present study highlights the importance of Dioscorea species as sources of diverse secondary metabolites for the isolation of active compound(s).
AB - Dioscorea pentaphylla L., a wild tuber is used both as food and medicines among different ethnic groups of Similipal Biosphere Reserve, India. Tubers are used against skin infections. In order to establish and confirm tribal claims, methanol extract was subjected to fractionation. The active fraction (DP1) was subsequently used for further purification and NMR (Nuclear magnetic resonance) characterization. The phytochemical analysis revealed the presence of saponin groups. The antibacterial activity of DP1 was done against selected bacterial strains (Salmonella typhi, Shigella flexneri, Streptococcus pyogenes, Streptococcus mutans and Vibrio cholerae) using DD (disc diffusion), AWD (agar well diffusion) and broth dilution assay. The activity was compared with antibiotics Penicillin and Kanamycin. It was observed that DP1 showed significant inhibitory activity against the tested bacteria. The characterization of DP1 through NMR analysis and presence of proton in carbon position at C-3, C-19, C-18, C-21 and C-27 was same as the known compound “Diosgenin”. Therefore, isolated compound was confirmed to be Diosgenin. The study for the first time showed that, diosgenin present in D. pentaphylla tuber was responsible for antibacterial and antioxidant potential. Present study highlights the importance of Dioscorea species as sources of diverse secondary metabolites for the isolation of active compound(s).
KW - Dioscorea pentaphylla/ antibacterial activity/antioxidant
KW - Dioscorea pentaphylla/extract/active fraction
KW - NMR characterization
UR - http://www.scopus.com/inward/record.url?scp=85045187247&partnerID=8YFLogxK
U2 - 10.1590/s2175-97902017000417006
DO - 10.1590/s2175-97902017000417006
M3 - Article
AN - SCOPUS:85045187247
SN - 1984-8250
VL - 53
JO - Brazilian Journal of Pharmaceutical Sciences
JF - Brazilian Journal of Pharmaceutical Sciences
IS - 4
M1 - e17006
ER -