TY - JOUR
T1 - Antioxidant and cytoprotective activity of the olive leaf (Olea europaea L. var. Kalamata) extracts on the mouse embryonic fibroblast cell
AU - Ha, Ju Yeon
AU - Goo, Sun Young
AU - Sung, Jung Suk
AU - Shin, Han Seung
PY - 2009/8
Y1 - 2009/8
N2 - Oleuropein content of olive leaf extracts (OLE; ethanol extract) was evaluated by high performance liquid chromatography analysis. Oleuropein contents were 4.21±0.57, 3.92±0.43, 0.32±0.03, 5.76±0.32, and 32.47±0.25 mg/100 g for ethanol extract, and hexane, chloroform, ethyl acetate, and butanol fraction, respectively. The removal of DPPH free radical increased in OLE and all 5 fractions of OLE in a concentration dependent manner. In order to investigate the antioxidant effect of OLE in vitro, 80%(v/v) ethanol OLE, H2O2, or combined treatment of 80%(v/v) ethanol OLE and H2O2 were applied on mouse embryonic fibroblast (MEF) cells. Cells were damaged by oxidative stress decreased their viability followed by increasing concentration of H2O2, but co-treatment of OLE and H2O2 showed an increase in cell growth about 20% compare to the cells treated with H2O2. OLE suppresses cytotoxicity induced by H2O2 in dose dependent manner. OLE treatment on MEF cells was also examined by analyzing cell cycle and apoptotic rate using flow cytometry. Apoptotic and necrotic cell accumulation was decreased in addition of OLE to H2O2 compare to the oxidative damaged cells. Taken together, these results demonstrated that OLE suppresses cytotoxicity induced by H2O2 and protect cells against oxidative stress on MEF cells.
AB - Oleuropein content of olive leaf extracts (OLE; ethanol extract) was evaluated by high performance liquid chromatography analysis. Oleuropein contents were 4.21±0.57, 3.92±0.43, 0.32±0.03, 5.76±0.32, and 32.47±0.25 mg/100 g for ethanol extract, and hexane, chloroform, ethyl acetate, and butanol fraction, respectively. The removal of DPPH free radical increased in OLE and all 5 fractions of OLE in a concentration dependent manner. In order to investigate the antioxidant effect of OLE in vitro, 80%(v/v) ethanol OLE, H2O2, or combined treatment of 80%(v/v) ethanol OLE and H2O2 were applied on mouse embryonic fibroblast (MEF) cells. Cells were damaged by oxidative stress decreased their viability followed by increasing concentration of H2O2, but co-treatment of OLE and H2O2 showed an increase in cell growth about 20% compare to the cells treated with H2O2. OLE suppresses cytotoxicity induced by H2O2 in dose dependent manner. OLE treatment on MEF cells was also examined by analyzing cell cycle and apoptotic rate using flow cytometry. Apoptotic and necrotic cell accumulation was decreased in addition of OLE to H2O2 compare to the oxidative damaged cells. Taken together, these results demonstrated that OLE suppresses cytotoxicity induced by H2O2 and protect cells against oxidative stress on MEF cells.
KW - Antioxidant activity
KW - Apoptosis
KW - Cell viability
KW - Oleuropein
KW - Olive leaf
UR - http://www.scopus.com/inward/record.url?scp=79954574092&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:79954574092
SN - 1226-7708
VL - 18
SP - 965
EP - 970
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
IS - 4
ER -