Antioxidant properties of Korean black raspberry wines and their apoptotic effects on cancer cells

Jaewook Jung, Mi Young Son, Seungwon Jung, Pilwoo Nam, Jung Suk Sung, Seung Joo Leec, Kwang Geun Lee

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

BACKGROUND: The objective of the study was to determine the antioxidant activities of Korean black raspberry concentrate (KBR: Rubus coreanus Miq.) and Korean black raspberry wine concentrates (KBRW-1 and KBRW-2) using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The apoptotic effects of the KBRW concentrates on various cancer cells were also investigated. RESULTS: Both the KBR concentrate and KBRW concentrates showed dose-related antioxidant activities in the DPPH assay. At concentrations of 500 μg mL -1 and 1000 μg mL-1, the KBR concentrate antioxidant activities were 59% and 79%, respectively. KBRW-1 and KBRW-2 inhibited radical formation at 500 μgm L-1 by 19% and 48%, respectively. The antioxidant activity of KBRW-2 was comparable to various red wines (RW-1, RW-2, RW-3, and RW-4). However, at 1000 μg mL-1, the antioxidant activities of KBRW-1 and KBRW-2 were 53% and 83% that of α-tocopherol, respectively. Both RW-1 and KBRW-2 showed marked antiproliferative effects on all cancer cell types, with decreases in cell viability greater than 50%. This was co-related with apoptotic characteristics, including chromat in condensation and apoptotic bodyformation, asdetermined by cellmorphological assessment. Cell cycle analysis showed that treating AGS cells with RW-1 and KBRW-2 resulted in 25% and 30% apoptotic cell accumulation, respectively. KBRW-2 induced the cleavage of poly(ADP-ribose) polymerase (PARP) and the activation of caspase-3 within the AGS cells. Levels of PARP cleavage were increased five- and three-fold by RW-1 and KBRW-2, respectively, and the level of caspase-3 was significantly increased by the treatment with KBRW-2. CONCLUSION: Overall, the results of this study suggest that Korean black raspberry wine displays antioxidant activity comparable to that of imported red wines, and has anticancer effects that may be attributed to induction of the apoptotic pathway.

Original languageEnglish
Pages (from-to)970-977
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume89
Issue number6
DOIs
StatePublished - Apr 2009

Keywords

  • Anticancer
  • Antioxidants
  • DPPH
  • Korean black raspberry wine
  • Lipid/MA

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