Apoptosis in leukemic cells induced by anti-proliferative coumarin isolated from the stem bark of fraxinus rhynchophylla

Beom Zoo Lee, Ik Soo Lee, Chau Ha Pham, Soon Kyu Jeong, Sulhae Lee, Kwang Won Hong, Hee Min Yoo

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Esculetin 6-O-β-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (EAG) is a coumarin glycoside isolated from the stem bark of Fraxinus rhynchophylla. This study scrutinized the anti-proliferative activity of EAG on blood cancer-derived Jurkat leukemic cells. Cell viability assays in leukemic cancer cells determined that EAG possesses potent anti-proliferative effects. Moreover, treatment with EAG increased the proportion of apoptotic cells, resulted in cell cycle arrest being induced at the subG0/ G1 phase, and reduced the proportion of cells present in the S phase. In addition, mitochondrial membrane potential was reduced by EAG in Jurkat cells. Additionally, EAG triggered apoptosis that was mediated by the downregulation of BCL-XL, p-IκBα, and p-p65 expressions in addition to the upregulation of cleaved Caspase 3 and BAX expressions. These findings revealed that the toxic effect of EAG was mediated by intracellular signal transduction pathways that involved a mechanism in which reactive oxygen species (ROS) were upregulated. Thus, this study concludes that EAG could potentially serve as a therapeutic agent for leukemia.

Original languageEnglish
Pages (from-to)1214-1221
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume30
Issue number8
DOIs
StatePublished - Aug 2020

Keywords

  • Apoptosis
  • Coumarin
  • Cytotoxicity
  • Fraxinus rhynchophylla
  • Leukemia
  • ROS

Fingerprint

Dive into the research topics of 'Apoptosis in leukemic cells induced by anti-proliferative coumarin isolated from the stem bark of fraxinus rhynchophylla'. Together they form a unique fingerprint.

Cite this