Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays

Hee Seok Lee; Eun Jung Park; Songyi Han; Gyeong Yong Oh; Hui Seung Kang; Jin Hyang Suh; Min Ki Shin; Hyun Suk Oh; Myung Sil Hwang; Guiim Moon; Young Ho Koh; Yooheon Park; Jin Hwan Hong; Yong Eui Koo

doi:10.1016/j.chemosphere.2017.10.084

Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays

Hee Seok Lee, Eun Jung Park, Songyi Han, Gyeong Yong Oh, Hui Seung Kang, Jin Hyang Suh, Min Ki Shin, Hyun Suk Oh, Myung Sil Hwang, Guiim Moon, Young Ho Koh, Yooheon Park, Jin Hwan Hong, Yong Eui Koo

Department of Food Science & Biotechnology

Korean Ministry of Food and Drug Safety

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on various chemicals, which are used in household products including cleaning agents and wetted tissues by in vitro OECD test guideline No. 458 (using AR-EcoScreen™ cell line) and the me-too test method (using 22Rv1cell line), which was adopted as OECD project No. 4.99. All chemicals were not determined as AR agonists. However α-dodecyl-ω-hydroxypoly (oxyethylene) and 3-iodo-2-propynyl butylcarbamate have shown a weak AR antagonistic effects with IC₅₀ values of 2.18 ± 0.12 and 4.26 ± 0.17 μg/ml via binding affinity to AR in only 22Rv1/mouse mammary tumor virus using AR transcriptional activation assay, because of their different cytotoxicity on each applied cell line. This report firstly provides information about agonistic/antagonistic effects against human AR of various chemicals including surfactants and biocides by OECD in vitro stably transfected transcriptional activation assays. However, further in vivo and human model studies are needed to confirm their adverse effects.

Original language	English
Pages (from-to)	589-596
Number of pages	8
Journal	Chemosphere
Volume	191
DOIs	https://doi.org/10.1016/j.chemosphere.2017.10.084
State	Published - Jan 2018

Keywords

Agonist
Antagonist
Human androgen receptor
OECD test guideline
Transcriptional activation assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.chemosphere.2017.10.084

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Lee, H. S., Park, E. J., Han, S., Oh, G. Y., Kang, H. S., Suh, J. H., Shin, M. K., Oh, H. S., Hwang, M. S., Moon, G., Koh, Y. H., Park, Y., Hong, J. H., & Koo, Y. E. (2018). Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays. Chemosphere, 191, 589-596. https://doi.org/10.1016/j.chemosphere.2017.10.084

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title = "Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays",

abstract = "The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on various chemicals, which are used in household products including cleaning agents and wetted tissues by in vitro OECD test guideline No. 458 (using AR-EcoScreen{\texttrademark} cell line) and the me-too test method (using 22Rv1cell line), which was adopted as OECD project No. 4.99. All chemicals were not determined as AR agonists. However α-dodecyl-ω-hydroxypoly (oxyethylene) and 3-iodo-2-propynyl butylcarbamate have shown a weak AR antagonistic effects with IC50 values of 2.18 ± 0.12 and 4.26 ± 0.17 μg/ml via binding affinity to AR in only 22Rv1/mouse mammary tumor virus using AR transcriptional activation assay, because of their different cytotoxicity on each applied cell line. This report firstly provides information about agonistic/antagonistic effects against human AR of various chemicals including surfactants and biocides by OECD in vitro stably transfected transcriptional activation assays. However, further in vivo and human model studies are needed to confirm their adverse effects.",

keywords = "Agonist, Antagonist, Human androgen receptor, OECD test guideline, Transcriptional activation assay",

author = "Lee, {Hee Seok} and Park, {Eun Jung} and Songyi Han and Oh, {Gyeong Yong} and Kang, {Hui Seung} and Suh, {Jin Hyang} and Shin, {Min Ki} and Oh, {Hyun Suk} and Hwang, {Myung Sil} and Guiim Moon and Koh, {Young Ho} and Yooheon Park and Hong, {Jin Hwan} and Koo, {Yong Eui}",

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year = "2018",

month = jan,

doi = "10.1016/j.chemosphere.2017.10.084",

language = "English",

volume = "191",

pages = "589--596",

journal = "Chemosphere",

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Lee, HS, Park, EJ, Han, S, Oh, GY, Kang, HS, Suh, JH, Shin, MK, Oh, HS, Hwang, MS, Moon, G, Koh, YH, Park, Y, Hong, JH & Koo, YE 2018, 'Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays', Chemosphere, vol. 191, pp. 589-596. https://doi.org/10.1016/j.chemosphere.2017.10.084

TY - JOUR

T1 - Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays

AU - Lee, Hee Seok

AU - Park, Eun Jung

AU - Han, Songyi

AU - Oh, Gyeong Yong

AU - Kang, Hui Seung

AU - Suh, Jin Hyang

AU - Shin, Min Ki

AU - Oh, Hyun Suk

AU - Hwang, Myung Sil

AU - Moon, Guiim

AU - Koh, Young Ho

AU - Park, Yooheon

AU - Hong, Jin Hwan

AU - Koo, Yong Eui

PY - 2018/1

Y1 - 2018/1

N2 - The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on various chemicals, which are used in household products including cleaning agents and wetted tissues by in vitro OECD test guideline No. 458 (using AR-EcoScreen™ cell line) and the me-too test method (using 22Rv1cell line), which was adopted as OECD project No. 4.99. All chemicals were not determined as AR agonists. However α-dodecyl-ω-hydroxypoly (oxyethylene) and 3-iodo-2-propynyl butylcarbamate have shown a weak AR antagonistic effects with IC50 values of 2.18 ± 0.12 and 4.26 ± 0.17 μg/ml via binding affinity to AR in only 22Rv1/mouse mammary tumor virus using AR transcriptional activation assay, because of their different cytotoxicity on each applied cell line. This report firstly provides information about agonistic/antagonistic effects against human AR of various chemicals including surfactants and biocides by OECD in vitro stably transfected transcriptional activation assays. However, further in vivo and human model studies are needed to confirm their adverse effects.

AB - The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on various chemicals, which are used in household products including cleaning agents and wetted tissues by in vitro OECD test guideline No. 458 (using AR-EcoScreen™ cell line) and the me-too test method (using 22Rv1cell line), which was adopted as OECD project No. 4.99. All chemicals were not determined as AR agonists. However α-dodecyl-ω-hydroxypoly (oxyethylene) and 3-iodo-2-propynyl butylcarbamate have shown a weak AR antagonistic effects with IC50 values of 2.18 ± 0.12 and 4.26 ± 0.17 μg/ml via binding affinity to AR in only 22Rv1/mouse mammary tumor virus using AR transcriptional activation assay, because of their different cytotoxicity on each applied cell line. This report firstly provides information about agonistic/antagonistic effects against human AR of various chemicals including surfactants and biocides by OECD in vitro stably transfected transcriptional activation assays. However, further in vivo and human model studies are needed to confirm their adverse effects.

KW - Agonist

KW - Antagonist

KW - Human androgen receptor

KW - OECD test guideline

KW - Transcriptional activation assay

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U2 - 10.1016/j.chemosphere.2017.10.084

DO - 10.1016/j.chemosphere.2017.10.084

M3 - Article

C2 - 29073568

AN - SCOPUS:85032877298

SN - 0045-6535

VL - 191

SP - 589

EP - 596

JO - Chemosphere

JF - Chemosphere

ER -

Assessment of androgen receptor agonistic/antagonistic effects on 25 chemicals in household applicants by OECD in vitro stably transfected transcriptional activation assays

Abstract

Keywords

UN SDGs

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