Assessment of chilling injury and molecular marker analysis in cucumber cultivars (Cucumis sativus L.)

Asjad Ali, Eun Mi Yang, Sun Woong Bang, Sang Min Chung, Jack E. Staub

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to 4°C (Experiment 1) and 1°C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and 149 μmoles;.m-2.s-1 photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling was 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalHorticultural Science and Technology
Volume32
Issue number2
DOIs
StatePublished - 2014

Keywords

  • Abiotic stress
  • Chloroplast genotypes
  • DNA marker
  • Low temperature
  • SdCAPS

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