Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays

Hee Seok Lee; Na Yeon Kim; Young Sun Song; Gyeong Yong Oh; Da Woon Jung; Da Hyun Jeong; Hui Seung Kang; Hyun Sook Oh; Yooheon Park; Jeong Sup Hong; Yong Eui Koo

doi:10.1016/j.tiv.2019.02.003

Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays

Hee Seok Lee, Na Yeon Kim, Young Sun Song, Gyeong Yong Oh, Da Woon Jung, Da Hyun Jeong, Hui Seung Kang, Hyun Sook Oh, Yooheon Park, Jeong Sup Hong, Yong Eui Koo

Department of Food Science & Biotechnology

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14 Scopus citations

Abstract

The presence of veterinary drug residues in foods and the environment could potentially cause adverse effects on humans and wildlife. Several veterinary drugs were reported to exhibit endocrine disrupting effects via binding affinities to sexual hormone receptors such as estrogen and androgen receptors. Therefore, we confirmed the human estrogen receptor (ER) agonistic/antagonistic effects of 135 chemicals that were used as veterinary drugs in Korea by the official Organization for Economic Cooperation and Development (OECD) in vitro ER transcriptional activation (TA) assay using the VM7Luc4E2 cell line. In the case of ER agonist screening, 7 veterinary drugs (cefuroxime, cymiazole, trenbolone, zeranol, phoxim, altrenogest and nandrolone) were determined to be ER agonists. In addition, only zeranol was found to exhibit weak ER antagonistic activity. These 7 veterinary drugs, which were determined as ER agonists and/or antagonists by an OECD in vitro assay, were also found to have binding affinity to ERs. These results indicate that various veterinary drugs possess potential (anti-)estrogenic effects. However, further study is needed to determine the precise endocrine-disrupting effects of these compounds.

Original language	English
Pages (from-to)	256-263
Number of pages	8
Journal	Toxicology in Vitro
Volume	58
DOIs	https://doi.org/10.1016/j.tiv.2019.02.003
State	Published - Aug 2019

Keywords

Agonist
Antagonist
Human estrogen receptor
OECD test guideline
Veterinary drug

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10.1016/j.tiv.2019.02.003

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Lee, H. S., Kim, N. Y., Song, Y. S., Oh, G. Y., Jung, D. W., Jeong, D. H., Kang, H. S., Oh, H. S., Park, Y., Hong, J. S., & Koo, Y. E. (2019). Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays. Toxicology in Vitro, 58, 256-263. https://doi.org/10.1016/j.tiv.2019.02.003

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title = "Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays",

abstract = "The presence of veterinary drug residues in foods and the environment could potentially cause adverse effects on humans and wildlife. Several veterinary drugs were reported to exhibit endocrine disrupting effects via binding affinities to sexual hormone receptors such as estrogen and androgen receptors. Therefore, we confirmed the human estrogen receptor (ER) agonistic/antagonistic effects of 135 chemicals that were used as veterinary drugs in Korea by the official Organization for Economic Cooperation and Development (OECD) in vitro ER transcriptional activation (TA) assay using the VM7Luc4E2 cell line. In the case of ER agonist screening, 7 veterinary drugs (cefuroxime, cymiazole, trenbolone, zeranol, phoxim, altrenogest and nandrolone) were determined to be ER agonists. In addition, only zeranol was found to exhibit weak ER antagonistic activity. These 7 veterinary drugs, which were determined as ER agonists and/or antagonists by an OECD in vitro assay, were also found to have binding affinity to ERs. These results indicate that various veterinary drugs possess potential (anti-)estrogenic effects. However, further study is needed to determine the precise endocrine-disrupting effects of these compounds.",

keywords = "Agonist, Antagonist, Human estrogen receptor, OECD test guideline, Veterinary drug",

author = "Lee, {Hee Seok} and Kim, {Na Yeon} and Song, {Young Sun} and Oh, {Gyeong Yong} and Jung, {Da Woon} and Jeong, {Da Hyun} and Kang, {Hui Seung} and Oh, {Hyun Sook} and Yooheon Park and Hong, {Jeong Sup} and Koo, {Yong Eui}",

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year = "2019",

month = aug,

doi = "10.1016/j.tiv.2019.02.003",

language = "English",

volume = "58",

pages = "256--263",

journal = "Toxicology in Vitro",

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Lee, HS, Kim, NY, Song, YS, Oh, GY, Jung, DW, Jeong, DH, Kang, HS, Oh, HS, Park, Y, Hong, JS & Koo, YE 2019, 'Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays', Toxicology in Vitro, vol. 58, pp. 256-263. https://doi.org/10.1016/j.tiv.2019.02.003

Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays. / Lee, Hee Seok; Kim, Na Yeon; Song, Young Sun et al.
In: Toxicology in Vitro, Vol. 58, 08.2019, p. 256-263.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays

AU - Lee, Hee Seok

AU - Kim, Na Yeon

AU - Song, Young Sun

AU - Oh, Gyeong Yong

AU - Jung, Da Woon

AU - Jeong, Da Hyun

AU - Kang, Hui Seung

AU - Oh, Hyun Sook

AU - Park, Yooheon

AU - Hong, Jeong Sup

AU - Koo, Yong Eui

PY - 2019/8

Y1 - 2019/8

N2 - The presence of veterinary drug residues in foods and the environment could potentially cause adverse effects on humans and wildlife. Several veterinary drugs were reported to exhibit endocrine disrupting effects via binding affinities to sexual hormone receptors such as estrogen and androgen receptors. Therefore, we confirmed the human estrogen receptor (ER) agonistic/antagonistic effects of 135 chemicals that were used as veterinary drugs in Korea by the official Organization for Economic Cooperation and Development (OECD) in vitro ER transcriptional activation (TA) assay using the VM7Luc4E2 cell line. In the case of ER agonist screening, 7 veterinary drugs (cefuroxime, cymiazole, trenbolone, zeranol, phoxim, altrenogest and nandrolone) were determined to be ER agonists. In addition, only zeranol was found to exhibit weak ER antagonistic activity. These 7 veterinary drugs, which were determined as ER agonists and/or antagonists by an OECD in vitro assay, were also found to have binding affinity to ERs. These results indicate that various veterinary drugs possess potential (anti-)estrogenic effects. However, further study is needed to determine the precise endocrine-disrupting effects of these compounds.

AB - The presence of veterinary drug residues in foods and the environment could potentially cause adverse effects on humans and wildlife. Several veterinary drugs were reported to exhibit endocrine disrupting effects via binding affinities to sexual hormone receptors such as estrogen and androgen receptors. Therefore, we confirmed the human estrogen receptor (ER) agonistic/antagonistic effects of 135 chemicals that were used as veterinary drugs in Korea by the official Organization for Economic Cooperation and Development (OECD) in vitro ER transcriptional activation (TA) assay using the VM7Luc4E2 cell line. In the case of ER agonist screening, 7 veterinary drugs (cefuroxime, cymiazole, trenbolone, zeranol, phoxim, altrenogest and nandrolone) were determined to be ER agonists. In addition, only zeranol was found to exhibit weak ER antagonistic activity. These 7 veterinary drugs, which were determined as ER agonists and/or antagonists by an OECD in vitro assay, were also found to have binding affinity to ERs. These results indicate that various veterinary drugs possess potential (anti-)estrogenic effects. However, further study is needed to determine the precise endocrine-disrupting effects of these compounds.

KW - Agonist

KW - Antagonist

KW - Human estrogen receptor

KW - OECD test guideline

KW - Veterinary drug

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U2 - 10.1016/j.tiv.2019.02.003

DO - 10.1016/j.tiv.2019.02.003

M3 - Article

C2 - 30742918

AN - SCOPUS:85061390529

SN - 0887-2333

VL - 58

SP - 256

EP - 263

JO - Toxicology in Vitro

JF - Toxicology in Vitro

ER -

Assessment of human estrogen receptor agonistic/antagonistic effects of veterinary drugs used for livestock and farmed fish by OECD in vitro stably transfected transcriptional activation assays

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Keywords

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