Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

Vivek K. Bajpai, Yuvraj Haldorai, Imran Khan, Sonam Sonwal, Mahendra Pal Singh, Seema Yadav, Bilal Ahamad Paray, Basit Latief Jan, Sung Min Kang, Yun Suk Huh, Young Kyu Han, Shruti Shukla

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

An ultrasensitive electrochemical immunosensor has been prepared using an immunofunctionalized zirconium (Zr)-based metal–organic framework (MOF) with gold (Au) decoration Au@UiO-66(NH2) composite-coated glassy carbon electrode (GCE) for the determination of infectious hepatitis B surface antigen (HBsAg). We fabricated GCE with specific composite via immune-functionalization using anti-HBsAg with Au nanoparticles embedded in UiO-66(NH2). The electrochemical sensing performance of the immunofunctionalized Au@UiO-66(NH2)/GCE with HBsAg was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, there was a linear dynamic relationship in the buffer system between the electrical signal and HBsAg levels over the range 1.13 fg mL−1–100 ng mL−1 (R2 = 0.999) with a detection limit of 1.13 fg mL−1. The total analysis time was 15 min per sample. Further validations were performed with HBsAg-spiked human serum samples, and similar detection limits as in the buffer system were observed with reduced signal intensities at lower concentrations of HBsAg (1, 10, and 100 fg mL−1) and minimal interference. The HBsAg electrochemical immunosensing assay had good selectivity and excellent reproducibility, thereby indicating its significant potential in the super-fast diagnosis of hepatitis B. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
Article number365
JournalMicrochimica Acta
Volume188
Issue number11
DOIs
StatePublished - Nov 2021

Keywords

  • Au nanoparticles
  • Composite glassy carbon electrode
  • Electrochemical sensing
  • HBsAg
  • Hepatitis B
  • Immunofunctionalized Au@UiO-66(NH)
  • Immunosensor
  • Voltammetry

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