Abstract
An ultrasensitive electrochemical immunosensor has been prepared using an immunofunctionalized zirconium (Zr)-based metal–organic framework (MOF) with gold (Au) decoration Au@UiO-66(NH2) composite-coated glassy carbon electrode (GCE) for the determination of infectious hepatitis B surface antigen (HBsAg). We fabricated GCE with specific composite via immune-functionalization using anti-HBsAg with Au nanoparticles embedded in UiO-66(NH2). The electrochemical sensing performance of the immunofunctionalized Au@UiO-66(NH2)/GCE with HBsAg was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, there was a linear dynamic relationship in the buffer system between the electrical signal and HBsAg levels over the range 1.13 fg mL−1–100 ng mL−1 (R2 = 0.999) with a detection limit of 1.13 fg mL−1. The total analysis time was 15 min per sample. Further validations were performed with HBsAg-spiked human serum samples, and similar detection limits as in the buffer system were observed with reduced signal intensities at lower concentrations of HBsAg (1, 10, and 100 fg mL−1) and minimal interference. The HBsAg electrochemical immunosensing assay had good selectivity and excellent reproducibility, thereby indicating its significant potential in the super-fast diagnosis of hepatitis B. Graphical abstract: [Figure not available: see fulltext.].
Original language | English |
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Article number | 365 |
Journal | Microchimica Acta |
Volume | 188 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2021 |
Keywords
- Au nanoparticles
- Composite glassy carbon electrode
- Electrochemical sensing
- HBsAg
- Hepatitis B
- Immunofunctionalized Au@UiO-66(NH)
- Immunosensor
- Voltammetry