BACTEC Korean Source Micro Scan Korean Source Phoenix Korean Source

Translated title of the contribution: Evaluation of MicroScan and phoenix system for rapid identification and susceptibility testing using direct inoculation from positive BACTEC blood culture bottles

Jae Woo Chung, Hong Seon Jeon, Heungsup Sung, Mi Na Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Procedures for rapid identification and susceptibility testing by direct inoculation (DI) from positive blood culture bottles into an automated system have not been standardized. This study was purposed to evaluate DI from BACTEC 9240 blood culture system (BD, USA) into MicroScan (Dade Behring, USA) or Phoenix (BD, USA). Methods: From May to June 2006, bacterial pellets from positive aerobic bottles showing grampositive cocci (GPC) or gram-negative rods (GNR) of single morphology were directly inoculated to MicroScan PosCombo1A and NegCombo32 and to Phoenix PMIC/ID-107 and NMIC/ID-53. In addition, the automated instruments were also inoculated from subcultures (standard inoculations, SI). Species identification and susceptibilities were compared between DI and SI and between MicroScan and Phoenix. Results: A total of 108, 104, and 78 specimens were tested with MicroScan, Phoenix, and both, respectively. When DI and SI were matched, 94.8% of GPC were correctly identified with MicroScan, compared to 80.7% with Phoenix, and 93.9% of GNR were correctly identified with MicroScan, compared to 95.7% with Phoenix. DI with MicroScan and Phoenix showed correct susceptibilities in 94.6% of 1,150 and 96.5% of 660 tests (with very major error [VME] of 1.1% and 1.1%), respectively, among GPC and in 94.4% of 942 and 96.3% of 781 tests (with VME of 0.6% and 0%), respectively, of GNR. Correlation of identification/susceptibilities between MicroScan and Phoenix using DI were 81.8%/98.0% for Staphylococcus aureus and 100.0%/95.6% for Escherichia coli. Conclusions: DI warrants a reliable method for identification and susceptibility testing of both GPC and GNR in MicroScan, and those of only GNR in Phoenix.

Translated title of the contributionEvaluation of MicroScan and phoenix system for rapid identification and susceptibility testing using direct inoculation from positive BACTEC blood culture bottles
Original languageKorean
Pages (from-to)25-34
Number of pages10
JournalKorean Journal of Laboratory Medicine
Volume29
Issue number1
DOIs
StatePublished - 2009

Keywords

  • Blood culture
  • Direct inoculation
  • Identification
  • MicroScan
  • Phoenix
  • Susceptibility

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