Abstract
Nanoparticle-based systems have significant prospective for diagnosis, treatment and prevention of tuberculosis (TB). The properties of nanoparticles enable improvement of drug bioavailability and reduction of the dosing frequency, and may resolve the problem of nonadherence to prescribed therapy, which is one of the major obstacles in the control of TB epidemics. In this study, the stable Ag nanoparticles were chemically synthesized and their shape and size distribution were characterized by Field Emission Scanning Electron Microscope (FESEM). The free-radical generation effect of Ag nanoparticles on microbial growth inhibition was investigated by UV visible spectroscopy. The antimicrobial activity of Ag nanoparticles was investigated against the standard reference strains of Mycobacterium tuberculosis H37Rv (ATCC 27294), M. smegmatis mc2155 with chloroform assay and gram-negative bacterium Escherichia coli (ATCC 10536) and Bacillus cereus (ATCC 99815) on solid media. As results, E. coli (ATCC 10536) and B. ceres (ATCC 99815) was inhibited at the low concentration of Ag nanoparticles, whereas the complete growth-inhibitory effects of M. tuberculosis H37Rv (ATCC 27294) and M. smegmatis mc2155 were further acquired after the treatment of chloroform. These results suggest that Ag nanoparticles can be used as effective growth inhibitors against E. coli (ATCC 10536), and B. cereus (ATCC 99815), M. tuberculosis H37Rv (ATCC 27294) and M. smegmatis mc2155 making them applicable to diverse medical devices to prevent infection and targeted drug delivery as is a potential candidate against mycobacteria by conjugating with other chemical entities as a therapeutic index.
Original language | English |
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Pages (from-to) | 282-287 |
Number of pages | 6 |
Journal | Journal of Bionanoscience |
Volume | 7 |
Issue number | 3 |
DOIs | |
State | Published - Jun 2013 |
Keywords
- AgNP
- B.cereus
- E.coli
- M.smegmatis
- Mycobacterium tuberculosis
- Silver nanoparticles