Biodegradation of diazo dye Direct brown MR by Acinetobacter calcoaceticus NCIM 2890

Gajanan Ghodake, Sheetal Jadhav, Vishal Dawkar, Sanjay Govindwar

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Acinetobacter calcoaceticus was employed for the degradation of Direct brown MR (DBMR), commercially used azo dye in the textile industry in order to analyze mechanism of the degradation and role of inhibitors, redox mediators and stabilizers of lignin peroxidase during decolorization. Induction of intracellular and extracellular lignin peroxidase, intracellular laccase and DCIP reductase represented their involvement in the biodegradation of DBMR. Decolorization and biodegradation of azo dye DBMR in broth were monitored by UV-visible spectrophotometer and TLC. The products obtained from A. calcoaceticus degradation were characterized by FTIR and identified by GC/MS as biphenyl amine, biphenyl, 3-amino 6-hydroxybenzoic acid and naphthalene diazonium. Germination (%) and growth efficiency of Sorghum vulgare and Phaseolus mungo seeds revealed the degradation of DBMR into less toxic products than original dye. A. calcoaceticus also has a potential to degrade diverse dyes present in the textile effluent, into nontoxic metabolites, hence A. calcoaceticus can be applied for the commercial application.

Original languageEnglish
Pages (from-to)433-439
Number of pages7
JournalInternational Biodeterioration and Biodegradation
Volume63
Issue number4
DOIs
StatePublished - Jun 2009

Keywords

  • A. calcoaceticus
  • Biodegradation
  • DBMR
  • Lignin peroxidase
  • Sorghum vulgare

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