TY - JOUR
T1 - Biofabrication of streptomycin-conjugated calcium phosphate nanoparticles using red ginseng extract and investigation of their antibacterial potential
AU - Das, Gitishree
AU - Baek, Kwang Hyun
AU - Patra, Jayanta Kumar
N1 - Publisher Copyright:
© 2019 Das et al.
PY - 2019/6
Y1 - 2019/6
N2 - Conjugation of nanoparticles (NPs) with antibiotics for treating multidrug resistant pathogens has been enormously studied now a days. In the current investigation, calcium phosphate (CaP) NPs were produced by co-precipitation using red ginseng extract as the reducing agent and were conjugated to the antibiotic streptomycin to form streptomycin-conjugated NPs (CPG-S NPs). The CPG-S NPs antibacterial activity was evaluated in this study against eight plant and five foodborne pathogenic bacteria. The synthesized CPG-S NPs were characterized by UV-VIS spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric and differential thermogravimetric analysis. CPG-S NPs exhibited promising antibacterial activity against all eight plant pathogenic bacteria and three of the five foodborne pathogenic bacteria tested; the diameter of inhibition zones ranged between 9.74-16.95 mm and 9.82-15.84 mm, respectively. CPG-S NPs displayed 50-100 μg/mL of minimum inhibitory concentration and 100 μg/mL of minimum bactericidal concentration against the plant and foodborne pathogenic bacterial strains, respectively. Furthermore, the SEM image of bacteria treated with CPG-S NPs displayed cells with a ruptured cell wall and fewer cells compared to the SEM image of untreated control bacteria displaying uniform and intact cells. SEM confirmed that CPG-S NPs degraded the bacterial cell wall and membrane resulting in lysed bacterial cells. In conclusion, the results suggest that CPG-S NPs could be effectively utilized in formulating drugs to treat bacterial plant or dental diseases and in manufacturing dental products such as toothpaste, mouthwashes, and artificial teeth.
AB - Conjugation of nanoparticles (NPs) with antibiotics for treating multidrug resistant pathogens has been enormously studied now a days. In the current investigation, calcium phosphate (CaP) NPs were produced by co-precipitation using red ginseng extract as the reducing agent and were conjugated to the antibiotic streptomycin to form streptomycin-conjugated NPs (CPG-S NPs). The CPG-S NPs antibacterial activity was evaluated in this study against eight plant and five foodborne pathogenic bacteria. The synthesized CPG-S NPs were characterized by UV-VIS spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, and thermogravimetric and differential thermogravimetric analysis. CPG-S NPs exhibited promising antibacterial activity against all eight plant pathogenic bacteria and three of the five foodborne pathogenic bacteria tested; the diameter of inhibition zones ranged between 9.74-16.95 mm and 9.82-15.84 mm, respectively. CPG-S NPs displayed 50-100 μg/mL of minimum inhibitory concentration and 100 μg/mL of minimum bactericidal concentration against the plant and foodborne pathogenic bacterial strains, respectively. Furthermore, the SEM image of bacteria treated with CPG-S NPs displayed cells with a ruptured cell wall and fewer cells compared to the SEM image of untreated control bacteria displaying uniform and intact cells. SEM confirmed that CPG-S NPs degraded the bacterial cell wall and membrane resulting in lysed bacterial cells. In conclusion, the results suggest that CPG-S NPs could be effectively utilized in formulating drugs to treat bacterial plant or dental diseases and in manufacturing dental products such as toothpaste, mouthwashes, and artificial teeth.
UR - http://www.scopus.com/inward/record.url?scp=85067177799&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0217318
DO - 10.1371/journal.pone.0217318
M3 - Article
C2 - 31181070
AN - SCOPUS:85067177799
SN - 1932-6203
VL - 14
JO - PLoS ONE
JF - PLoS ONE
IS - 6
M1 - e0217318
ER -