TY - JOUR
T1 - Biotransformation enzymes in Cunninghamella blakesleeana (NCIM-687)
AU - Bhosale, Sanjyot
AU - Saratale, Ganesh
AU - Govindwar, Sanjay
PY - 2006
Y1 - 2006
N2 - Presence of higher enzyme levels of aminopyrine N-demethylase, aniline hydroxylase and 11-β hydroxylase activities were observed in Cunninghamella blakesleeana grown in potato-dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and showed inhibition with CO, indicating cytochrome P450 mediated reactions. A significant increase in aniline hydroxylase enzyme activity was observed when mycelia incubated in incubation medium containing different inducers (viz. camphor, cholesterol, naphthalene, veratrole, phenobarbital, n-hexadecane and ethyl alcohol) when compared with mycelia incubated in same way but in absence of inducers. Cunninghamella blakesleeana (NCIM 687) have shown the ability to degrade cholesterol, camphor and naphthalene when 96 h grown mycelia incubated in incubation medium containing these organic compounds.
AB - Presence of higher enzyme levels of aminopyrine N-demethylase, aniline hydroxylase and 11-β hydroxylase activities were observed in Cunninghamella blakesleeana grown in potato-dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and showed inhibition with CO, indicating cytochrome P450 mediated reactions. A significant increase in aniline hydroxylase enzyme activity was observed when mycelia incubated in incubation medium containing different inducers (viz. camphor, cholesterol, naphthalene, veratrole, phenobarbital, n-hexadecane and ethyl alcohol) when compared with mycelia incubated in same way but in absence of inducers. Cunninghamella blakesleeana (NCIM 687) have shown the ability to degrade cholesterol, camphor and naphthalene when 96 h grown mycelia incubated in incubation medium containing these organic compounds.
UR - http://www.scopus.com/inward/record.url?scp=33845625741&partnerID=8YFLogxK
U2 - 10.1002/jobm.200510117
DO - 10.1002/jobm.200510117
M3 - Article
C2 - 17139609
AN - SCOPUS:33845625741
SN - 0233-111X
VL - 46
SP - 444
EP - 448
JO - Journal of Basic Microbiology
JF - Journal of Basic Microbiology
IS - 6
ER -