Biotransformation enzymes in Cunninghamella blakesleeana (NCIM-687)

Sanjyot Bhosale, Ganesh Saratale, Sanjay Govindwar

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Presence of higher enzyme levels of aminopyrine N-demethylase, aniline hydroxylase and 11-β hydroxylase activities were observed in Cunninghamella blakesleeana grown in potato-dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and showed inhibition with CO, indicating cytochrome P450 mediated reactions. A significant increase in aniline hydroxylase enzyme activity was observed when mycelia incubated in incubation medium containing different inducers (viz. camphor, cholesterol, naphthalene, veratrole, phenobarbital, n-hexadecane and ethyl alcohol) when compared with mycelia incubated in same way but in absence of inducers. Cunninghamella blakesleeana (NCIM 687) have shown the ability to degrade cholesterol, camphor and naphthalene when 96 h grown mycelia incubated in incubation medium containing these organic compounds.

Original languageEnglish
Pages (from-to)444-448
Number of pages5
JournalJournal of Basic Microbiology
Volume46
Issue number6
DOIs
StatePublished - 2006

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