C-Myc amplification altered the gene expression of ABC-and SLC-transporters in human breast epithelial cells

Keon Wook Kang, Young Bin Im, Woon Jung Go, Hyo Kyung Han

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The present study aimed to investigate the effect of c-Myc overexpression on the gene expression and functional activity of major drug transporters in breast epithelial cells. In order to reflect c-Myc overexpressed epithelial cells, MCF10A cells (human mammary epithelial cells) stably expressing c-Myc (c-Myc-MCF10A cells) have been established by retroviral infection and then the effect of c-Myc activation on the gene expression and functional activity of 16 influx and efflux transporters was examined in c-Myc-MCF10A cells. In RT-PCR analysis, thequantitative difference in transporters' gene expression profiles was observed after c-Myc activation. Western blot analysis also indicated that the protein expression of some transporters (MCT1, OCT1, MRP1, MRP2 and BCRP) increased by c-Myc overexpression. In parallel to the alteration in gene expression, functional activity studies indicated that the cellular uptake of benzoic acid, MPP+, calcein-AM, H33342, representative substrates of MCT1, OCT1, MRPs and BCRP, respectively, was significantly (p < 0.05) altered by c-Myc overexpression. In conclusion, c-Myc amplification appeared to be coupled with the modulation of gene expression of certain drug transporters in human breast epithelial cells.

Original languageEnglish
Pages (from-to)627-633
Number of pages7
JournalMolecular Pharmaceutics
Volume6
Issue number2
DOIs
StatePublished - 6 Apr 2009

Keywords

  • C-Myc
  • Gene expression
  • Human breast epithelial cells
  • Regulation
  • Transporters

Fingerprint

Dive into the research topics of 'C-Myc amplification altered the gene expression of ABC-and SLC-transporters in human breast epithelial cells'. Together they form a unique fingerprint.

Cite this