Cadmium-induced biomarkers discovery and comparative network analysis in Daphnia magna

Jun Hyuek Yang; Hyo Jeong Kim; Sang Min Lee; Bo Mi Kim; Young Rok Seo

doi:10.1007/s13273-017-0036-3

Cadmium-induced biomarkers discovery and comparative network analysis in Daphnia magna

Jun Hyuek Yang, Hyo Jeong Kim, Sang Min Lee, Bo Mi Kim, Young Rok Seo

Department of Life Science

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Exposure to cadmium is gradually increased and its toxicity can be fatal to organisms in aquatic environment. D. magna inhabits in water. It is recommended by OECD guideline to test toxicity of chemicals. Through acute immobilization test, we observed a mobility change of D. magna caused by cadmium chloride exposure. The EC50 value of cadmium chloride was 14.5 ppb. Using D. magna in EC50 value of cadmium, we conducted microarray analysis to investigate differential gene expression and found 247 up- and 146 down-regulated genes. We performed BLAST analysis to identify and understand the meaning of the microarray data in comparative genomics. Comparing common proteins in some species, potential biomarkers and functions are discovered through pathway analysis. Based on differential gene expression of cadmium-treated D. magna, potential biomarkers are identified and investigated by functional network of mammals including human through comparative genomic analysis.

Original language	English
Pages (from-to)	327-336
Number of pages	10
Journal	Molecular and Cellular Toxicology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/s13273-017-0036-3
State	Published - 1 Sep 2017

Keywords

Biomarker
BLAST
Cadmium
Comparative genomic study
Daphnia magna
Microarray

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13273-017-0036-3

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title = "Cadmium-induced biomarkers discovery and comparative network analysis in Daphnia magna",

abstract = "Exposure to cadmium is gradually increased and its toxicity can be fatal to organisms in aquatic environment. D. magna inhabits in water. It is recommended by OECD guideline to test toxicity of chemicals. Through acute immobilization test, we observed a mobility change of D. magna caused by cadmium chloride exposure. The EC50 value of cadmium chloride was 14.5 ppb. Using D. magna in EC50 value of cadmium, we conducted microarray analysis to investigate differential gene expression and found 247 up- and 146 down-regulated genes. We performed BLAST analysis to identify and understand the meaning of the microarray data in comparative genomics. Comparing common proteins in some species, potential biomarkers and functions are discovered through pathway analysis. Based on differential gene expression of cadmium-treated D. magna, potential biomarkers are identified and investigated by functional network of mammals including human through comparative genomic analysis.",

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author = "Yang, {Jun Hyuek} and Kim, {Hyo Jeong} and Lee, {Sang Min} and Kim, {Bo Mi} and Seo, {Young Rok}",

note = "Publisher Copyright: {\textcopyright} 2017, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media B.V.",

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doi = "10.1007/s13273-017-0036-3",

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T1 - Cadmium-induced biomarkers discovery and comparative network analysis in Daphnia magna

AU - Yang, Jun Hyuek

AU - Kim, Hyo Jeong

AU - Lee, Sang Min

AU - Kim, Bo Mi

AU - Seo, Young Rok

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Exposure to cadmium is gradually increased and its toxicity can be fatal to organisms in aquatic environment. D. magna inhabits in water. It is recommended by OECD guideline to test toxicity of chemicals. Through acute immobilization test, we observed a mobility change of D. magna caused by cadmium chloride exposure. The EC50 value of cadmium chloride was 14.5 ppb. Using D. magna in EC50 value of cadmium, we conducted microarray analysis to investigate differential gene expression and found 247 up- and 146 down-regulated genes. We performed BLAST analysis to identify and understand the meaning of the microarray data in comparative genomics. Comparing common proteins in some species, potential biomarkers and functions are discovered through pathway analysis. Based on differential gene expression of cadmium-treated D. magna, potential biomarkers are identified and investigated by functional network of mammals including human through comparative genomic analysis.

AB - Exposure to cadmium is gradually increased and its toxicity can be fatal to organisms in aquatic environment. D. magna inhabits in water. It is recommended by OECD guideline to test toxicity of chemicals. Through acute immobilization test, we observed a mobility change of D. magna caused by cadmium chloride exposure. The EC50 value of cadmium chloride was 14.5 ppb. Using D. magna in EC50 value of cadmium, we conducted microarray analysis to investigate differential gene expression and found 247 up- and 146 down-regulated genes. We performed BLAST analysis to identify and understand the meaning of the microarray data in comparative genomics. Comparing common proteins in some species, potential biomarkers and functions are discovered through pathway analysis. Based on differential gene expression of cadmium-treated D. magna, potential biomarkers are identified and investigated by functional network of mammals including human through comparative genomic analysis.

KW - Biomarker

KW - BLAST

KW - Cadmium

KW - Comparative genomic study

KW - Daphnia magna

KW - Microarray

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DO - 10.1007/s13273-017-0036-3

M3 - Article

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SP - 327

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JO - Molecular and Cellular Toxicology

JF - Molecular and Cellular Toxicology

IS - 3

ER -

Cadmium-induced biomarkers discovery and comparative network analysis in Daphnia magna

Abstract

Keywords

UN SDGs

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