Caffeine-loaded gold nanoparticles conjugated with PLA-PEG-PLA copolymer for in vitro cytotoxicity and anti-inflammatory activity

Reena Kamalakannan, Gajendiran Mani, Prabakaran Muthusamy, Arul Antony Susaimanickam, Kyobum Kim

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The purpose of the study was to develop caffeine-loaded gold nanoparticles (AuNPs) using a poly(lactic acid)-polyethylene glycol-poly(lactic acid) (PLA-PEG-PLA) polymer to enhance the anti-inflammatory activity of caffeine. Caffeine-loaded AuNPs were conjugated to PLA-PEG-PLA copolymer matrix via π-back bond between AuNPs and the ester carbonyl group of the polyester. The π-back bonded ester carbonyl oxygen strongly interacted with the caffeine molecule and exhibited enhanced anti-inflammatory activity. The physico-chemical characteristics of the resulting nanoconjugates were evaluated by a series of microscopic, diffraction, and spectroscopic methods. In vitro assays indicated increasing membrane stabilization of red blood cells and enhanced inhibition of protein denaturation.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalJournal of Industrial and Engineering Chemistry
Volume51
DOIs
StatePublished - 25 Jul 2017

Keywords

  • Anti-inflammatory activity
  • Caffeine
  • Gold nanoparticles
  • In vitro cytotoxicity
  • PLA-PEG-PLA copolymer

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