Cationic liposome-enhanced adenoviral gene transfer in a murine head and neck cancer model

Myung Whun Sung, Sang Goo Lee, Seong Jun Yoon, Hyun Joo Lee, Dae Seog Heo, Kwang Hyun Kim, Tae Yong Koh, Seung Ho Choi, Seok Won Park, Ja Won Koo, Tae Young Kwon

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The effect of combining adenoviral vector and cationic liposomes on the efficiency of gene transfer to head and neck tumor cells was investigated. Two human and two murine cell lines were used for the screening of gene transfer efficiency using an adenoviral vector. Cationic liposome-enhanced gene transfer was checked using a murine squamous carcinoma cell line, SCCVII/SF. A considerable difference in the efficiency of gene transduction was observed among the cell lines. The combination of DOSPER and adenoviral vector containing human alkaline phosphatase showed a remarkable enhancing effect in gene transfer in vitro and in vivo, compared to the adenovirus alone or control groups. With an improvement in the efficiency of gene transfer, it may be possible not only to enhance the expression of transduced genes, but also to deliver a smaller amount of virus, as a result, reducing toxicity and the immune response against adenovirus.

Original languageEnglish
Pages (from-to)1653-1656
Number of pages4
JournalAnticancer Research
Volume20
Issue number3 A
StatePublished - 2000

Keywords

  • Adenoviral vector
  • Gene transfer
  • Liposome

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