TY - JOUR
T1 - Changes after a month following micropulse cyclophotocoagulation in normal porcine eyes
AU - Ahn, So Min
AU - Choi, Mihyun
AU - Kim, Seong Woo
AU - Kim, Yong Yeon
N1 - Publisher Copyright:
© 2021 The Authors.
PY - 2021/11
Y1 - 2021/11
N2 - Purpose: To analyze the effects on the uvea, including the pars plicata and ciliary muscle, and retina in normal porcine eyes after performing micropulse transslceral cyclophoto-coagulation (MP-TSCPC) with the different energy levels, and conventional continuous wave transslceral cyclophotocoagulation. Methods: MP-TSCPC was performed in a total of 15 eyes at the different energy levels of 60 J, 120 J, 180 J, 240 J, and 300 J, respectively. Continuous wave transslceral cyclopho-tocoagulation was performed in three eyes and the other three eyes were controls. The eyes were enucleated after a month following the laser treatment and the uvea and retina were analyzed using hematoxylin and eosin staining and immunohistochemistry staining. Results: After MP-TSCPC 60 J, the expression of α-smooth muscle actin (α-SMA) and glial fibrillary acidic protein in the ciliary muscle was increased, although there was no structural change in pars plicata. After MP-TSCPC 120 J, partial destruction of the ciliary epithelium was observed in pars plicata, and the retinal thickness was increased. After MP-TSCPC 240 J and 300 J, the structural destruction of the pars plicata and ciliary muscle was observed, and the expression of α-SMA and glial fibrillary acidic protein in pars plicata and the expression of α-SMA in ciliary muscle were increased. Conclusions: Histologic changes in the uvea and peripheral retina were different based on the energy levels of MP-TSCPC. In particular, MP-TSCPC with low energy levels mainly affected the ciliary muscle, while MP-TSCPC with high energy levels affected both the ciliary muscle and pars plicata. Our results may imply a possibility of intraocular damage with MP-TSCPC in humans. Translational Relevance: Based on our research, it is possible to infer the possibility of intraocular damage in humans according to the different levels of energy in the clinic.
AB - Purpose: To analyze the effects on the uvea, including the pars plicata and ciliary muscle, and retina in normal porcine eyes after performing micropulse transslceral cyclophoto-coagulation (MP-TSCPC) with the different energy levels, and conventional continuous wave transslceral cyclophotocoagulation. Methods: MP-TSCPC was performed in a total of 15 eyes at the different energy levels of 60 J, 120 J, 180 J, 240 J, and 300 J, respectively. Continuous wave transslceral cyclopho-tocoagulation was performed in three eyes and the other three eyes were controls. The eyes were enucleated after a month following the laser treatment and the uvea and retina were analyzed using hematoxylin and eosin staining and immunohistochemistry staining. Results: After MP-TSCPC 60 J, the expression of α-smooth muscle actin (α-SMA) and glial fibrillary acidic protein in the ciliary muscle was increased, although there was no structural change in pars plicata. After MP-TSCPC 120 J, partial destruction of the ciliary epithelium was observed in pars plicata, and the retinal thickness was increased. After MP-TSCPC 240 J and 300 J, the structural destruction of the pars plicata and ciliary muscle was observed, and the expression of α-SMA and glial fibrillary acidic protein in pars plicata and the expression of α-SMA in ciliary muscle were increased. Conclusions: Histologic changes in the uvea and peripheral retina were different based on the energy levels of MP-TSCPC. In particular, MP-TSCPC with low energy levels mainly affected the ciliary muscle, while MP-TSCPC with high energy levels affected both the ciliary muscle and pars plicata. Our results may imply a possibility of intraocular damage with MP-TSCPC in humans. Translational Relevance: Based on our research, it is possible to infer the possibility of intraocular damage in humans according to the different levels of energy in the clinic.
KW - Ciliary muscle
KW - Micropulse
KW - Pars plicata
KW - Retina
KW - Transscleral cyclophotocoagulation
KW - Uvea
UR - http://www.scopus.com/inward/record.url?scp=85119114521&partnerID=8YFLogxK
U2 - 10.1167/tvst.10.13.11
DO - 10.1167/tvst.10.13.11
M3 - Article
C2 - 34751743
AN - SCOPUS:85119114521
SN - 2164-2591
VL - 10
JO - Translational Vision Science and Technology
JF - Translational Vision Science and Technology
IS - 13
M1 - 11
ER -