Changes after a month following micropulse cyclophotocoagulation in normal porcine eyes

So Min Ahn, Mihyun Choi, Seong Woo Kim, Yong Yeon Kim

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Purpose: To analyze the effects on the uvea, including the pars plicata and ciliary muscle, and retina in normal porcine eyes after performing micropulse transslceral cyclophoto-coagulation (MP-TSCPC) with the different energy levels, and conventional continuous wave transslceral cyclophotocoagulation. Methods: MP-TSCPC was performed in a total of 15 eyes at the different energy levels of 60 J, 120 J, 180 J, 240 J, and 300 J, respectively. Continuous wave transslceral cyclopho-tocoagulation was performed in three eyes and the other three eyes were controls. The eyes were enucleated after a month following the laser treatment and the uvea and retina were analyzed using hematoxylin and eosin staining and immunohistochemistry staining. Results: After MP-TSCPC 60 J, the expression of α-smooth muscle actin (α-SMA) and glial fibrillary acidic protein in the ciliary muscle was increased, although there was no structural change in pars plicata. After MP-TSCPC 120 J, partial destruction of the ciliary epithelium was observed in pars plicata, and the retinal thickness was increased. After MP-TSCPC 240 J and 300 J, the structural destruction of the pars plicata and ciliary muscle was observed, and the expression of α-SMA and glial fibrillary acidic protein in pars plicata and the expression of α-SMA in ciliary muscle were increased. Conclusions: Histologic changes in the uvea and peripheral retina were different based on the energy levels of MP-TSCPC. In particular, MP-TSCPC with low energy levels mainly affected the ciliary muscle, while MP-TSCPC with high energy levels affected both the ciliary muscle and pars plicata. Our results may imply a possibility of intraocular damage with MP-TSCPC in humans. Translational Relevance: Based on our research, it is possible to infer the possibility of intraocular damage in humans according to the different levels of energy in the clinic.

Original languageEnglish
Article number11
JournalTranslational Vision Science and Technology
Volume10
Issue number13
DOIs
StatePublished - Nov 2021

Keywords

  • Ciliary muscle
  • Micropulse
  • Pars plicata
  • Retina
  • Transscleral cyclophotocoagulation
  • Uvea

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