Abstract
A simple and sensitive electrochemical sensor for the simultaneous detection of dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA) was developed, based on the poly(thionine) (PTH) modified glassy carbon electrode (GCE). The PTH/GCE sensor could clearly separate the electro-oxidation signals of DA and UA from that of AA. The accumulated surface charges of the PTH film and pH of the base solutions were optimized for improved simultaneous quantitative detection of DA and UA. The oxidation peak currents of DA and UA were both linear over the range from 5 to 30 μM in the presence of 200 μM AA. The sensor showed sensitivities of 22.87 and 6.02 μA/cm2/μM with detection limits (S/N=3) of 0.7 and 1.3 μM for DA and UA, respectively. The sensor obtained interference free signals and excellent recoveries from spiked human urine samples for both DA and UA detection.
Original language | English |
---|---|
Pages (from-to) | 7806-7815 |
Number of pages | 10 |
Journal | International Journal of Electrochemical Science |
Volume | 8 |
Issue number | 6 |
State | Published - Jun 2013 |
Keywords
- Differential pulse voltammetry
- Dopamine
- Poly(thionine)
- Simultaneous detection
- Uric acid